3-Methyl-L-Histidine Analysis Service

3-Methyl-L-Histidine (3-MH) is a metabolite mainly produced during the degradation of muscle proteins and is an important indicator of the muscle protein breakdown rate. Since dietary protein contains low levels of 3-MH, its concentration in urine and blood primarily reflects muscle protein metabolism. Analyzing 3-MH levels is crucial for studying muscle protein metabolism, diagnosing muscle diseases, and assessing nutritional status.

 

Changes in 3-MH levels can be associated with various physiological and pathological conditions, such as muscle atrophy, exercise-induced damage, malnutrition, and certain metabolic diseases. Accurate measurement of 3-MH concentrations provides insights into muscle protein metabolism dynamics, offering a scientific basis for clinical diagnosis and treatment.

 



Figure 1. Molecular Structure of 3-MH

 

MtoZ Biolabs utilizes liquid chromatography-tandem mass spectrometry (LC-MS/MS) to conduct detailed quantitative and qualitative analyses of 3-MH. This advanced method integrates LC's rapid separation capabilities with the high detection sensitivity of MS/MS, facilitating the precise measurement of 3-MH in complex biological matrices.

 

Analysis Workflow

 

 

 

Service Advantages

 



 

Sample Submission Requirements

Please ensure that the sample volume is greater than 0.2 g or 0.2 mL.

 

Applications

Clinical Diagnosis: Detect levels in patients with muscle atrophy, exercise-induced damage, and malnutrition to assist in diagnosis and prognosis.

 

Metabolic Research: Investigate the role and metabolic pathways of 3-MH in muscle protein degradation.

 

Drug Development: Evaluate the impact of drugs on 3-MH metabolism to aid in developing new therapeutic agents.

 

Nutritional Research: Analyze the metabolic changes of 3-MH under different dietary conditions, providing scientific evidence for nutritional regulation and dietary supplementation.

 

Deliverables

1. Experimental Procedures

2. Relevant Liquid Chromatography and Mass Spectrometry Parameters

3. Detailed Information on 3-MH

4. Raw Data

5. Custom Analysis Report

 

Our 3-Methyl-L-Histidine analysis service provides high-quality data support, advancing your scientific research and clinical studies. For any inquiries or requirements, please contact us.