Lipidomics Analysis Service
Lipidomics is a study of composition and quantification of lipids at a systematic level, so as to uncover the interactions between lipids and other macromolecules, and the mechanisms of lipids in regulating biological activities. At present, LC-MS and shotgun lipidomics are two mainstream technology for lipidomics analysis. LC-MS utilizes high-throughput liquid chromatography system to separate lipids based on their physical and chemical properties, followed by mass spectrometry analysis. Whereas, shotgun lipidomics is independent of liquid chromatography technology, and separates lipids through intrasource separation system, which adjusts pH value of solution and changes the ionization status of lipids, followed by direct infusion into mass spectrometer for lipids identification.
Figure 1. A Few Common Types of Lipids
LC-MS is a powerful tool for discovery lipidomics, as LC-MS usually achieves detection of lipids at ppm level and accurate analysis of lipids side chains through MS2 fragmentation. Whereas, shotgun lipidomics is more widely used for (semi)-quantitation of targeted lipids. MtoZ Biolabs provides both LC-MS and shotgun lipidomics services to meet your specific needs.
Analysis Workflow
Service Advantages
1. Professional and Customized Experimental Design to Meet Your Research Requirements
2. Multiple Choices of State-of-the-Art Analytical Platforms for Reaching a Higher Level Both in Depth and Scope
3. Lower Sample Demand
4. Stringent Quality Control System Covering the Entire Analytical Processes, Ensuring Acquisition of High-Quality Data
5. Compatible with a Variety of Types of Samples, Ranging from All Kinds of Tissues and Cell Samples to Body Fluids, Etc.
Sample Submission Requirements
Note: We will perform testing experiments before we start official experiments. To ensure the most cost-effective and accurate analysis is provided, only qualified samples will proceed to the official analysis.
Deliverables
1. Experiment Procedures
2. Parameters of Liquid Chromatography and Mass Spectrometer
3. MS Raw Data Files
4. MS Data Quality Checks
5. Metabolites Quantification Data
6. Bioinformatics Analysis (PCA, KEGG, etc)
Related Services
Metabolomics
Quantitative Proteomics
Protein Analysis
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Oxylipids, bioactive molecules derived from the oxidation of unsaturated fatty acids like linolenic, arachidonic, and eicosatetraenoic acids, encompass compounds such as prostaglandins, leukotrienes, and various enols and ketones. Oxylipidomics, a burgeoning field, delves into the composition, distribution, transformation, and function of oxylipids in biological systems. Given their pivotal roles in cellular signaling and disease pathogenesis,
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• Lipidomics MALDI Imaging Service
MALDI-IMS is a powerful tool for examining the distribution of lipids in tissues. As an ion source, MALDI is particularly well-suited for thin tissue sections and is extensively employed for analysis of tissue samples. The benefits of MALDI-IMS include: 1) Simplified extraction steps; 2) Reduced sample loss; 3) Omission of complex deduction processes; 4) Provision of detailed spatial organization and distribution data.
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Extracellular vesicles (EVs), prevalent in a multitude of body fluids and cell culture supernatants, are secreted by most cellular types. These include exosomes, which are diminutive vesicles characterized by a lipid bilayer with diameters ranging from 50 to 150 nm. The genesis of exosomes involves several stages: invagination of the plasma membrane, endosome formation, progression to multivesicular bodies (MVBs), and eventual extracellular release.
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• Targeted Lipidomics Analysis Service
Lipids, small molecules that are either hydrophobic or amphipathic, include fats, waxes, sterols, and fat-soluble vitamins (A, D, E, and K), monoacylglycerides, diacylglycerides, and phospholipids. In biological physiology, lipids play crucial roles not only in energy storage and structural components of cellular membranes but also in facilitating signal transduction, membrane transporting, and morphogenesis.
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• Untargeted Lipidomics Analysis Service
Lipids are the main components of biological membranes. Lipids are not only the main form of energy storage in living cells but also serve as mediators of cell signaling. The lipidome consists of 8 major classes, over 80 main categories, 300 subclasses, and thousands of lipids at different concentrations. Lipidomics is the systematic study of all lipid molecules (> 30,000 species) within a biological system, tissue, body fluid, or cell.
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