Resources

Proteomics Databases



Metabolomics Databases

• • How Does Lactate Drive Histone Lactylation in Gene Regulation?

  Lactate is not merely an intermediate of energy metabolism; it also functions as a signaling molecule, profoundly influencing gene expression through histone lactylation. This discovery reshapes our understanding of lactate and offers new perspectives in oncology, immunology, and metabolic disease research. Histones are the core proteins that package DNA within the nucleus, regulating gene accessibility and expression through various covalent modifications, including acetylation, methylation, and phos......

• • How to Enrich Low-Abundance Histone Kbu Peptides?

  In epigenetic research, histone modifications are recognized as critical molecular markers that regulate gene expression, chromatin architecture, and cell fate. Among these, histone lysine butyrylation (Kbu, Lysine Butyrylation), a novel acetylation-derived modification, has garnered increasing attention. Histone Kbu plays pivotal roles in metabolism, stem cell differentiation, and disease pathogenesis; however, its low abundance and dynamic nature present significant challenges for accurate detection......

• • Which Antibody Is Optimal for Histone Butyrylation Enrichment?

  Histone acetylation, methylation, and other post-translational modifications have long been central to epigenetics research. Histone butyrylation (Histone Butyrylation, HBu), a novel short-chain fatty acylation modification, is increasingly attracting attention from both academia and the biopharmaceutical industry. Accurate investigation of HBu dynamics is critical for understanding gene regulation, metabolic states, and disease mechanisms. In histone modification analyses, the enrichment step is pivo......

• • How to Isolate Golgi Fractions for Subcellular Proteomics?

  In subcellular proteomics, the Golgi apparatus has long attracted significant attention as a central hub for intracellular protein processing and sorting. Its essential roles in protein glycosylation, vesicular trafficking, and the secretory pathway make it a critical target for elucidating disease mechanisms and identifying potential therapeutic targets. However, precise isolation of the Golgi apparatus remains technically challenging because of its highly dynamic architecture and its extensive physi......

• • Histone Methylation Quantitative Proteomics by LC-MS/MS

  Quantitative LC-MS/MS analysis of histone methylation sites including mono-, di-, and tri-methyl states with antibody enrichment.

• • Quantitative Enzyme Activity Analysis by ABPP and LC-MS/MS

  Quantify active enzymes with activity-based probes, enrichment, and LC-MS/MS using TMT or label-free workflows.

• • Label-Free Quantitative Proteomics for Protein Interactions

  Label-free LC-MS/MS quantification applied to Co-IP and interaction proteomics without isotope tags.

• • Absolute vs Relative Quantitative Proteomics Explained

  Compare absolute and relative quantitative proteomics: isotope standards, SWATH, label-free, and TMT/iTRAQ methods.

• • How to Use GFP Tags for Co-Immunoprecipitation?

  GFP-tag Co-IP using GFP-Trap beads, gentle lysis, wash optimization, and optional LC-MS/MS for interactome discovery.

• • Chemoproteomics: Principles, Workflow, and Applications

  Chemoproteomics uses activity-based probes and LC-MS to map protein–small-molecule interactions, targets, and enzyme activity.