Targeted Proteomics Service

Based on mass spectrometry analysis technology, combined with prior bioinformatics analysis, the Targeted Proteomics Service utilizes techniques such as Multiple Reaction Monitoring (MRM) or Selected Reaction Monitoring (SRM) to accurately target specific peptides and ion signals for quantitative analysis. Unlike non-targeted proteomics, this approach selectively chooses ions for the collection of mass spectrometry data, enabling the analysis of numerous samples with enhanced accuracy, sensitivity, and reproducibility. Targeted proteomics is applicable to a range of studies including post-translational modifications, protein conformations, protein-protein interactions, dynamics, and the systemic investigation of metabolic and signaling pathways.

 



Chen, Y. et al. Methods Mol Biol. 2019.

Figure1. A Typical Pipeline of Targeted Proteomics

 

Targeted proteomics quantitative techniques mainly include multiple reaction monitoring (MRM) and parallel reaction monitoring (PRM). MRM, also referred to as selected reaction monitoring (SRM), employs triple quadrupole mass spectrometry (QQQ-MS) to capture signals conforming to predefined ion criteria, facilitating both relative and absolute quantification of target proteins. PRM extends the capabilities of MRM by integrating the high selectivity of quadrupoles with the superior resolution and precision of the Orbitrap technology. It measures all fragment ions following the fragmentation of selected parent ions, offering improved detection sensitivity and reduced susceptibility to interference.

 



Figure 2. Schematic Diagram of the MRM

 

 



 Figure 3. Schematic Diagram of the PRM

 

Services at MtoZ Biolabs

MtoZ Biolabs offers the Targeted Proteomics Service based on high-precision mass spectrometry technology, focusing on providing reliable quantitative analysis of target proteins for our clients. Using advanced MRM and SRM techniques, we are able to precisely quantify specific target proteins and their peptides, greatly enhancing the sensitivity and accuracy of the analysis. Our service can efficiently detect low-abundance proteins in complex biological samples and handle a wide variety of sample types, including serum, tissues, and cell extracts, to meet diverse research needs.

 

The Targeted Proteomics Service is one of the core technologies at MtoZ Biolabs in the field of proteomics. With advanced mass spectrometry techniques and extensive industry experience, we are committed to providing high-quality, customized proteomics analysis to support the success of our clients' research and discoveries, which include:

1. Parallel Reaction Monitoring (PRM) Services

2. MRM/PRM-Based Quantitative Proteomics Analysis

3. Absolute Quantification Analysis （AQUA）

 

Why Choose MtoZ Biolabs?

1. Advance Analysis Platform: MtoZ Biolabs established an advanced targeted proteomics analysis platform, guaranteeing reliable, fast, and highly accurate analysis service.

2. One-Time-Charge: Our pricing is transparent, no hidden fees or additional costs.

3. High-Data-Quality: Deep data coverage with strict data quality control. AI-powered bioinformatics platform integrate targeted proteomics analysis data providing clients with a comprehensive data report.

4. Antibody-Independent Detection: MRM/PRM does not require antibodies, broadening its applicability in analyzing complex biological samples while avoiding the specificity limitations of antibody-based methods.

5. Customized Service: Tailored to the specific research needs of our clients, we offer flexible experimental design and personalized data analysis to ensure the achievement of research goals to the fullest extent.

 

 Sample Submission Requirements

 



 

Applications

1. Screening and Validation of Disease Biomarkers

Targeted Proteomics Service is particularly important in disease research. By quantitatively analyzing specific proteins in samples such as blood, urine, and tissues, researchers can identify biomarkers associated with specific diseases, providing robust data support for early diagnosis, prognostic assessment, and therapeutic monitoring. In studies of various diseases, including cancer, cardiovascular diseases, and neurodegenerative diseases, Targeted Proteomics Service has become an indispensable tool.

 

2. Drug Development and Precision Medicine

In drug development, Targeted Proteomics Service can be used for screening and validating target proteins, helping to identify drug action targets and their mechanisms. Additionally, with the rise of precision medicine, there is an increasing demand for protein analysis tailored to personalized treatments. Targeted Proteomics Service provides reliable data to support the development of personalized treatment plans.

 

3. Protein Function and Interaction Studies

Proteins play a crucial role in cellular functions and biological processes. Targeted Proteomics Service helps scientists quantitatively analyze the expression levels, post-translational modifications, and interactions of specific proteins, thus providing deep insights into the biological functions of proteins. Whether studying protein signal transduction, metabolic pathways, or cell cycle regulation, Targeted Proteomics Service offers precise data to support fundamental biological research.

 

Case Study

Targeted Proteomics Reveals Quantitative Differences in Low-Abundance Glycosyltransferases of Patients with Congenital Disorders of Glycosylation

Using targeted proteomics technology, particularly Multiple Reaction Monitoring (MRM), the study quantitatively analyzed the expression of low-abundance glycosyltransferases in patients with Congenital Disorders of Glycosylation (CDG). The researchers first selected key enzymes involved in glycosylation reactions and performed analysis using a liquid chromatography-mass spectrometry (LC-MS/MS) system. In MRM mode, the mass spectrometer selected specific parent and daughter ions, significantly enhancing the sensitivity and specificity of the analysis, enabling precise quantification of low-abundance proteins. This approach successfully revealed differences in the abundance of glycosyltransferases in serum and tissue samples from CDG patients, providing important data for understanding the pathogenesis of CDG and supporting the application of glycosyltransferases as potential biomarkers.

 



Sakson, R. et al. Int. J. Mol. Sci. 2024.

Figure4. Schematic Workflow for Establishment and Usage of the MRM Assay

Deliverables

What Could be Included in the Report?

1. Comprehensive Experimental Details

2. Materials, Instruments, and Methods

3. Total Ion Chromatogram & Quality Control Assessment

4. Data Analysis, Preprocessing, and Estimation

5. Bioinformatics Analysis

6. Raw Data Files

 

Targeted Proteomics Service is a highly precise, sensitive, and efficient protein quantification analysis service, widely used in fields such as disease research, drug development, and precision medicine. Through advanced mass spectrometry techniques and accurate analytical methods, MtoZ Biolabs is able to provide high-quality proteomics data to help clients gain deeper insights into the complex behaviors of proteins in biological processes and promote progress in research and applications in related fields. Whether in basic research or clinical applications, the Targeted Proteomics Service offers high-precision and efficient analytical solutions, meeting the diverse needs of modern life science research.