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    Protein Identification Service

      MtoZ Biolabs utilizes 2D-nano LC-MS/MS technology for protein identification. In our general workflow, protein complexes are separated in 1D/2D gel and the target protein is then digested into peptide fragments, followed by HPLC separation and tandem MS analysis. We use Scaffold and Mascot software for analyzing peptide MS data, and ensuring confident protein identification.

       

      Experimental Instruments

      image-Protein-Identification-workflow.png

       

      Sample Submission Requirements

      Format Gel bands, gel spots, and liquid samples are acceptable.
      Quantity Gel bands/spots that are visible to naked eyes are adequate for protein identification. For liquid samples, a total of 10 ug proteins are required.
      Purity Purity of liquid samples should be as high as possible. For liquid sample <10 ug, please avoid large amounts of detergent and salt ions, and note the buffer composition and the estimated amount of total proteins when submitting samples.
      Note All reagents/solvent used must be of the highest purity to reduce contaminating substances. Samples should be handled with extreme caution and always in clean condition. Any source that may introduce contaminating proteins should be eliminated.

       

      Deliverables

      1. Experiment Procedures

      2. Parameters of Liquid Chromatography and Mass Spectrometer

      3. MS Raw Data Files

      4. Peptide Identifications and Intensity

      5. Protein Identifications and Intensity

       

      Related Services

      Protein Analysis

      Protein Identification

      Protein Mass Measurement

      LC-MS Analysis of Pull-Down Proteins

      Native MS Analysis

       

      Protein Sequencing

      Protein De Novo Seq

      N-Terminal Sequencing

      C-Terminal Sequencing

      Edman Degradation

      Protein Full-Length Sequencing

       

      PTM Analysis

      Phospho Proteomics

      Acetyl Proteomics

      Ubiquitin Proteomics

      Glyco Proteomics

      Disulfide Bond

      Histone Modifications

    • • Accurate Mass Determination Service

      Accurate mass determination can be applied to all areas encompassed by chemistry and Accurate mass determination can be applied to all areas involved in chemistry and biochemistry. It is used to identify unknown compounds in complex mixtures, thereby simplifying the identification of all components. The accuracy of the determination refers to the degree of proximity between the measured value and its true value. In recent years, the mass determination of biological samples has received increasing attention,

    • • Protein Structure Identification Service

      The utilization of the bottom-up approach in proteomics is often constrained by the incomplete characterization of alternatively spliced isoforms, a wide array of post-translational modifications, and native protein degradation.

    • • Mass Spectrometry-Based Protein Identification Service

      The identification and analysis of single proteins or protein mixtures via mass spectrometry, as well as the analysis of individual protein sequences, are critical tasks in biological research. Techniques include determining protein molecular weights, sequencing protein N- and C-termini, analyzing protein sequences, and identifying post-translational modification sites.

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