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    Protein Identification Service

      MtoZ Biolabs utilizes 2D-nano LC-MS/MS technology for protein identification. In our general workflow, protein complexes are separated in 1D/2D gel and the target protein is then digested into peptide fragments, followed by HPLC separation and tandem MS analysis. We use Scaffold and Mascot software for analyzing peptide MS data, and ensuring confident protein identification.


      Experimental Instruments



      Sample Submission Requirements

      Format Gel bands, gel spots, and liquid samples are acceptable.
      Quantity Gel bands/spots that are visible to naked eyes are adequate for protein identification. For liquid samples, a total of 10 ug proteins are required.
      Purity Purity of liquid samples should be as high as possible. For liquid sample <10 ug, please avoid large amounts of detergent and salt ions, and note the buffer composition and the estimated amount of total proteins when submitting samples.
      Note All reagents/solvent used must be of the highest purity to reduce contaminating substances. Samples should be handled with extreme caution and always in clean condition. Any source that may introduce contaminating proteins should be eliminated.



      1. Experiment Procedures

      2. Parameters of Liquid Chromatography and Mass Spectrometer

      3. MS Raw Data Files

      4. Peptide Identifications and Intensity

      5. Protein Identifications and Intensity


      Related Services

      Protein Analysis

      Protein Identification

      Protein Mass Measurement

      LC-MS Analysis of Pull-Down Proteins

      Native MS Analysis


      Protein Sequencing

      Protein De Novo Seq

      N-Terminal Sequencing

      C-Terminal Sequencing

      Edman Degradation

      Protein Full-Length Sequencing


      PTM Analysis

      Phospho Proteomics

      Acetyl Proteomics

      Ubiquitin Proteomics

      Glyco Proteomics

      Disulfide Bond

      Histone Modifications

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