Protein Identification Service

MtoZ Biolabs utilizes 2D-nano LC-MS/MS technology for protein identification. In our general workflow, protein complexes are separated in 1D/2D gel and the target protein is then digested into peptide fragments, followed by HPLC separation and tandem MS analysis. We use Scaffold and Mascot software for analyzing peptide MS data, and ensuring confident protein identification.

Experimental Instruments

Sample Submission Requirements

Format	Gel bands, gel spots, and liquid samples are acceptable.
Quantity	Gel bands/spots that are visible to naked eyes are adequate for protein identification. For liquid samples, a total of 10 ug proteins are required.
Purity	Purity of liquid samples should be as high as possible. For liquid sample <10 ug, please avoid large amounts of detergent and salt ions, and note the buffer composition and the estimated amount of total proteins when submitting samples.
Note	All reagents/solvent used must be of the highest purity to reduce contaminating substances. Samples should be handled with extreme caution and always in clean condition. Any source that may introduce contaminating proteins should be eliminated.