Prokaryotic Transcriptomics Sequencing Service

The transcriptome is the complete set of mRNA within a cell under specific developmental stages or physiological conditions. Transcriptome sequencing, as a technical means of studying the transcriptome, is widely used in biological science research. Analyzing the transcriptome information of biological samples helps in studying the gene transcription situation and transcriptional regulation patterns within cells. In prokaryotes, the majority of RNA is rRNA, with mRNA accounting for only 1% to 5% of the total RNA. Therefore, sequencing the prokaryotic transcriptome requires purification of the mRNA. Additionally, prokaryotic mRNA, unlike eukaryotic mRNA, does not have a poly-A structure, so it cannot be directly purified using oligo-T. Generally, methods to remove rRNA are used to construct libraries for prokaryotic transcriptome sequencing. MtoZ Biolabs employs effective methods to remove rRNA from different prokaryotes, ensuring the quality of transcriptome sequencing.

 

 Advantages

1. Technology Advantages

MtoZ Biolabs' transcriptome sequencing service is based on the Illumina high-throughput sequencing platform, capable of detecting the overall transcription levels of any species at the single-nucleotide level. While analyzing the structure and expression levels of transcripts, it can also discover unknown and rare transcripts, and accurately identify alternative splicing sites and coding single nucleotide polymorphisms (cSNPs), thus providing the most comprehensive transcriptome information.

 

2. Service Advantages

(1) Experienced technicians provide a full range of professional services from experimental design, sample detection, data analysis, etc.

(2) Clear processes reduce unnecessary sample and time waste, with short delivery times.

(3) Autonomous biometrics analysis platform that provides advanced customized analysis in addition to routine analysis.

(4) MtoZ Biolabs has proteomics, metabolomics, and other analysis platforms, allowing for multi-omics integration analysis to enhance the quality of publications.

 

Analysis Workflow

 



Figure 1. Prokaryotic Transcriptome Sequencing Workflow

 

Applications



 

Case Study

 



Figure 2. MtoZ Biolabs Transcriptome Sequencing Case Display