ATR FT-IR Method for Analysis of Monosaccharide Composition

The ATR FT-IR method (Attenuated Total Reflectance Fourier Transform Infrared Spectroscopy) is an effective method for analyzing monosaccharide composition. This technique utilizes the infrared absorption characteristics of specific functional groups in monosaccharide molecules to identify and quantify monosaccharides.

 

Basic Principle

In ATR FT-IR analysis, infrared light passes through the sample, with some light being absorbed by the molecules in the sample and some being reflected. Molecules with different chemical structures absorb light at specific infrared wavelengths, and these absorption peaks can be used to identify specific compounds in the sample.

 

Monosaccharides such as glucose, fructose, etc. contain specific functional groups, such as hydroxyl (OH) and carbonyl (C=O), which have characteristic absorption in specific infrared bands. By analyzing these absorption peaks, the type and content of monosaccharides in the sample can be determined.

 

Technical Advantages

The ATR FT-IR method has the advantages of being fast, requiring no extensive sample preparation, and non-destructive. It can be used for rapid screening and analysis, making it particularly suitable for rapid analysis of multiple or complex samples.

 

This method is not only suitable for the analysis of monosaccharides, but also often used for the analysis of polysaccharides, glycoproteins, and other glyco-biomolecules. It has wide applications in food science, biotechnology, and the pharmaceutical industry.

 

Limitations

Although the ATR FT-IR method is very effective for monosaccharide analysis, it does have some limitations. For example, for monosaccharides with similar structures, their infrared absorption peaks may be very similar, which may require the combination of other analytical methods for accurate identification.