Glutamine Metabolism Analysis Service

Glutamine, a non-essential amino acid encoded by genetic sequences, features a unique side chain that converts to include an additional nitrogen atom, making it vital for numerous biochemical reactions. In cells, glutamine is synthesized from glutamate, catalyzed by glutamine synthetase in the cytoplasm. Additionally, its catabolism occurs in mitochondria, where it is converted to glutamate and ammonia, crucial intermediates for cellular energy and growth. These processes are essential for supporting nucleotide synthesis, neurotransmission, and also play roles in antioxidant protection and nitrogen detoxification, highlighting glutamine's critical function in maintaining cellular health and metabolic equilibrium. Consequently, analyzing glutamine metabolism thoroughly is crucial for understanding its biological roles comprehensively.

 



Figure 1. Molecular Structure of Glutamine

 

MtoZ Biolabs utilizes liquid chromatography-tandem mass spectrometry (LC-MS/MS) to conduct detailed quantitative and qualitative analyses of glutamine and its metabolites. This advanced method integrates LC's rapid separation capabilities with the high detection sensitivity of MS/MS, facilitating the precise measurement of glutamine in complex biological matrices.

 

Analysis Workflow

 



 

Services at MtoZ Biolabs

Targeted Metabolomics

Untargeted Metabolomics

Stable Isotope Labeling Experiments

Metabolic Flux Analysis

Metabolite Identification and Annotation

Data Analysis and Interpretation

 

Service Advantages

 



 

Sample Submission Requirements

 



 For detailed sample submission instructions, please consult our technical team.

 

Applications

Clinical Research: Monitors glutamine variations in different disease states, aiding in diagnosing and treating conditions such as liver disease, cancer, and metabolic disorders.

 

Nutritional Research: Measures glutamine levels in foods and supplements, informing dietary strategies and supplementation plans.

 

Sports Medicine: Evaluates changes in glutamine metabolism after physical activity, enhancing recovery and nutritional interventions.

 

Pharmaceutical Development: Investigates how prospective drugs affect glutamine pathways, supporting the innovation and assessment of new pharmacological treatments.

 

Deliverables

1. Experimental Procedures

2. Relevant Liquid Chromatography and Mass Spectrometry Parameters

3. Detailed Information on Metabolites

4. Raw Data

5. Custom Analysis Report