Histidine Analysis Service

Histidine, an essential amino acid, plays a critical role in numerous biological processes and is a vital component of proteins. It functions as a biochemical sensor through its unique imidazole side chain, responding to pH variations. The metabolic pathways of histidine, encompassing synthesis, degradation, and transformation into other amino acids, are crucial for normal physiological functions. Given that deviations in histidine levels are closely associated with various diseases, including metabolic disorders, neurodegenerative diseases, and immune deficiencies, precise histidine analysis is instrumental for biomarker identification in clinical diagnostics and research, enabling early disease detection and treatment strategy development.

 



Figure 1. Molecular Structure of Histidine

 

MtoZ Biolabs utilizes liquid chromatography-tandem mass spectrometry (LC-MS/MS) to conduct detailed quantitative and qualitative analyses of histidin and its metabolites. This advanced method integrates LC's rapid separation capabilities with the high detection sensitivity of MS/MS, facilitating the precise measurement of histidin in complex biological matrices.

 

Analysis Workflow

 

Services at MtoZ Biolabs

Standard Curve Construction

Ensures analytical accuracy and reliability.

 

Raw Data Preprocessing

Enhances the efficiency of data analysis through optimized workflows.

 

Histidine Quantification

Provides accurate measurements of histidine concentrations in samples.

 

Differential Metabolite Screening

Identifies metabolic alterations associated with diseases or physiological conditions.

 

Advanced Analytical Techniques

Employs methods such as KEGG pathway analysis and hierarchical clustering to elucidate the biological functions of histidine.

 

Service Advantages



 

Sample Submission Requirements

 

For detailed sample submission instructions, please consult our technical team.

 

Applications

Disease Diagnosis: Utilizes histidine level anomalies for diagnosing and treating metabolic disorders at early stages.

 

Nutritional Evaluation: Assesses nutritional status based on histidine levels, guiding dietary supplementation and adjustments.

 

Pharmacological Studies: Investigates the impact of histidine and its derivatives on drug development, improving research efficiency.

 

Biomarker Discovery: Leverages histidine levels as biomarkers for the early detection of diseases and monitoring of treatment efficacy.

 

MtoZ Biolabs is dedicated to advancing your research and clinical projects by providing detailed insights into histidin's extensive physiological roles through top-tier analytical techniques and expert services.