L-Proline Analysis Service

L-Proline is a natural amino acid essential for maintaining protein structure and function. It plays a crucial role in metabolism, acting as a central molecule in the proline metabolic pathway, where it can be converted to glutamate. This conversion is involved in various biochemical processes, including energy metabolism, cellular osmoregulation, and antioxidant responses.

 

Proline, a racemic mixture, contains L-proline, the biologically active form. Analyzing L-Proline helps in understanding protein structures and functions and in identifying changes in disease states such as tumors, liver diseases, and neurodegenerative conditions. Therefore, accurate analysis of L-proline is significant in biomedical research and clinical diagnostics.

 



Figure 1. Molecular Structure of L-Proline

 

MtoZ Biolabs utilizes liquid chromatography-tandem mass spectrometry (LC-MS/MS) to conduct detailed quantitative and qualitative analyses of L-proline and its metabolites. This advanced method integrates LC's rapid separation capabilities with the high detection sensitivity of MS/MS, facilitating the precise measurement of L-proline in complex biological matrices.

 

Analysis Workflow

 

 

 

Service Advantages

1. High Sensitivity and Precision

HPLC-MS/MS provides highly sensitive and accurate measurements, suitable for detecting low concentrations of L-proline and differentiating its isomers.

 

2. Rapid and Efficient

Optimized sample preparation and fast analysis ensure quick and efficient processing.

 

3. Wide Applicability

Suitable for various biological samples, including blood, urine, and tissues.

 

4. Accurate and Reliable Data

Ensures high reproducibility and low error rates, supporting both research and clinical applications, providing reliable and specific results.

 

Sample Submission Requirements

Sample Types

Serum, plasma, urine, tissue homogenates, and other biological samples.

 

Sample Volume

At least 200 μL of liquid sample or 200 mg of tissue sample.

 

Sample Preservation

Store and transport samples at -80°C to maintain L-proline stability.

 

Applications

Clinical Diagnosis: Detect L-proline levels in patients with liver diseases, tumors, and neurodegenerative diseases to aid in diagnosis and prognosis.

 

Metabolic Research: Investigate the role of L-proline in metabolic pathways and its interactions with other metabolites, revealing its functions in energy metabolism and cell protection.

 

Drug Development: Assess the impact of drugs on L-proline metabolism to aid in the development of new therapeutic drugs.

 

Nutritional Research: Analyze metabolic changes of L-proline under different dietary conditions, providing scientific evidence for nutritional regulation and dietary supplementation.

 

Environmental Research: Monitor the effects of environmental pollutants on L-proline levels in organisms to evaluate the biological impact of environmental pollution.

 

Deliverables

1. Experimental Procedures

2. Relevant Liquid Chromatography and Mass Spectrometry Parameters

3. Detailed Information on L-Proline and their Metabolites

4. Raw Data

5. Custom Analysis Report

 

MtoZ Biolabs is dedicated to advancing your research and clinical projects by providing detailed insights into L-proline's extensive physiological roles through top-tier analytical techniques and expert services.