Procyanidin B2 Analysis Service

Polyphenols are the largest group of secondary metabolites in plants, and their structural determination has been a focal point in research. Among polyphenols, procyanidin, also known as condensed tannins, are derived from proanthocyanidins. Procyanidin and proanthocyanidins are ubiquitously present in the plant kingdom and are commonly found in varying concentrations in everyday foods such as fruits, vegetables, legumes, grains, and nuts, as well as in cosmetics and pharmaceuticals containing plant materials. As natural antioxidants, procyanidins are used to stabilize food color, prevent rancidity caused by the oxidation of unsaturated fats, and for the chemoprevention of various degenerative tumors. Besides their antioxidant properties, procyanidin have reported anticancer, anti-infection, anti-inflammatory, cardioprotective, antibacterial, antimutagenic, wound healing, antidiabetic, and antiallergic activities.

 

Procyanidin B2 is formed by two epicatechin units connected via B-type bonds (C4-C8). It is commonly found in grape seeds, apple peels, chocolate, and red wine. This compound demonstrates antioxidative, anti-inflammatory, anticancer, and cardioprotective properties. Procyanidin B2 is extensively used in nutritional supplements, cosmetics, and pharmaceuticals.

 



Figure 1. The Structure of Procyanidin B2
 

 

Services at MtoZ Biolabs

1. Detectable Procyanidins at MtoZ Biolabs

 

 

 

2. Procyanidins Analysis Techniques

• High-Performance Liquid Chromatography (HPLC): For separating and quantifying different types of proanthocyanidins.
• Mass Spectrometry (MS): Combined with HPLC to provide molecular weight and structural information of proanthocyanidins.
• Nuclear Magnetic Resonance (NMR): For detailed molecular structure analysis of proanthocyanidins.
• Spectroscopy (e.g., UV-Vis and FTIR): For preliminary identification and quantitative analysis.

 

3. Procyanidin A1 Targeted Metabolomics Analysis

 



Figure 2. The Workflow of Targeted Metabolomics for Procyanidin B2
 

Applications

1. Antioxidant Activity

 



Zou, YP. et al. J Orthop Res. 2023.

 

2. Ameliorating the Progression of Osteoarthritis

 



Cai, W. et al. Int Immunopharmacol. 2022.

 

Proanthocyanidins, oligomeric compounds composed of catechin and epicatechin monomers, are widely present in food and hold significant medicinal value. Utilizing the LC-MS technology platform, MtoZ Biolabs provides qualitative and quantitative analysis of procyanidin B2 with exceptional sensitivity, reproducibility, and accuracy, expanding the research on its biological activities and medicinal value.

 

Deliverables

1. Experimental Procedures

2. Relevant Mass Spectrometry Parameters

3. Detailed Information on Procyanidin B2 Analysis

4. Mass Spectrometry Images

5. Raw Data