Peptide Mass Spectrometric Identification Service

Peptides, as pivotal biological molecules, are chains formed by amino acids linked through peptide bonds. Essential for both structural integrity and various biological functions—including hormone regulation, neurotransmission, and immune responses—peptides are critical in both health and disease.

 

MtoZ Biolabs is equipped with advanced mass spectrometry equipment and bioinformatics analysis tools. Combined with years of accumulated proteomics experience, we offer first-class peptide mass spectrometric identification services, achieving efficient separation and precise identification of peptides in biological samples.

 

Analysis Workflow

1. Sample Preparation

Proteins are extracted from samples and digested enzymatically into peptides.

 

2. Peptide Separation

High-performance liquid chromatography (HPLC) is employed to separate peptides.

 

3. Mass Spectrometry

Primary Analysis (MS): Determines the mass-to-charge ratios (m/z) of peptides.

Secondary Analysis (MS/MS): Further dissects selected peptides to decode their sequences.

 

4. Data Interpretation

Bioinformatics tools analyze MS/MS data against protein databases, identifying peptide sequences and modifications.

 



Figure 1. Analysis Workflow of Peptide Mass Spectrometric Identification

 

Service Advantages

Precision: Detects and precisely identifies peptides even at low concentrations.

Versatility: Effective across diverse sample types, from clinical to standard lab samples.

Efficiency: High-throughput capabilities ensure rapid processing and quick results.

Automation: Fully automated processes enhance both efficiency and accuracy.

 

Sample Submission Requirements

Types

Accepts liquid, solid, or gel forms.

 

Quantity

Minimum of 50 micrograms of protein required.

 

Storage

Samples must be kept frozen and protected from repeated freeze-thaw cycles prior to analysis.

 

Applications

·  Biomarker Discovery

·  Drug Design and Optimization

·  Proteomics Studies

·  Clinical Research

 

Deliverables

1. Experimental Procedures

2. Relevant Mass Spectrometry Parameters

3. Detailed Information on Peptides

4. Mass Spectrometry Images

5. Raw Data