Antibody C-terminal variation analysis
- High Sensitivity and Specificity: Mass spectrometry can accurately identify minute variations and modifications.
- Comprehensiveness: Capable of detecting multiple types of variations and modifications simultaneously.
- High Throughput: Enables the simultaneous analysis of a large number of samples, increasing efficiency.
- Quantitative Capability: Provides quantitative analysis of the abundance of variations and modifications.
Antibody C-terminal variation refers to the amino acid sequence variation in the C-terminal region of the heavy or light chain of the antibody. These variations can include amino acid substitutions, insertions, deletions, or modifications such as glycosylation and phosphorylation. Antibody C-terminal variation analysis provides detailed information on amino acid sequence variations and detects post-translational modifications in the C-terminal region, helping to understand the impact of these variations on the overall structure and function of the antibody.
Mengjing, Bao. et al. eLife. 2023.
Figure 1. Mass spectrometry Analysis of Glutamylation of the C-terminal domains of Proteins
Services at MtoZ Biolabs
Antibody C-terminal variation analysis is crucial in antibody research and development, offering critical technical support for the optimization and quality control of antibody drugs. MtoZ Biolabs provides comprehensive antibody C-terminal variation analysis services, addressing various issues encountered in antibody drug development, quality control, and function optimization. If you are interested in our services, please feel free to contact us.
Analysis Workflow
1. Sample Preparation: Extract antibodies from cell culture supernatant, serum, or purified antibody samples.
2. Enzymatic Digestion: Use specific proteases, such as trypsin, to cleave the antibodies, generating peptide fragments.
3. Mass Spectrometry Analysis: Detect and identify peptide sequences and modifications using liquid chromatography-tandem mass spectrometry (LC-MS/MS).
4. Data Analysis: Perform peptide matching and identify variations and modifications using specialized software and databases.
5. Result Validation: Confirm the presence of variations and modifications through further experiments such as Western blot or ELISA.
Service Advantages
Applications
Antibody Drug Development: Evaluate and optimize C-terminal variations of candidate antibodies to enhance drug efficacy and stability.
Biopharmaceutical Quality Control: Monitor the consistency of antibodies during production to ensure product quality.
Basic Research: Investigate the impact of C-terminal variations on antibody structure and function to uncover mechanisms of antibody action.
Clinical Research: Assess the performance of antibodies in clinical applications to ensure their safety and efficacy.
Deliverables
1. Experimental Procedures
2. Relevant Mass Spectrometry Parameters
3. Detailed Information on Antibody C-terminal Variation
4. Mass Spectrometry Images
5. Raw Data
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