Protein Full-Length Sequencing Sercive

Accurate determination of a protein's full-length sequence is essential in biopharmaceuticals and genetic engineering. Understanding the entire sequence from the N-terminus to the C-terminus is vital for comprehending a protein's biological functions within cells. In protein engineering and biopharmaceutical applications, precisely identifying the full sequence of therapeutic proteins is crucial for ensuring their bioactivity and functionality, providing key data for the development and optimization of treatment strategies.

 



Mai, Z. et al. Anal Chem. 2022.

Figure1. Protein Full-Length Sequencing

 

Services at MtoZ Biolabs

MtoZ Biolabs utilizes an innovative strategy for digesting proteins completely, employing a combination of enzymes such as Trypsin and Chymotrypsin to enhance peptide production and ensure comprehensive digestion. This approach enables high-coverage and high-precision sequence analysis in a single experiment, significantly improving peptide overlap and sequence completeness through the use of multiple enzymes and meticulous procedural steps. This method guarantees accurate protein identification and sequencing even in complex samples.

 

Analysis Workflow

1. Sequence Prediction

Based on the client's provided gene sequence, predict the protein structure and select appropriate proteases.

2. Enzymatic Digestion

Employ specially chosen enzymes to digest the protein, ensuring a diverse array of protein fragments.

3. High-Performance Liquid Chromatography-Tandem Mass Spectrometry (HPLC-MS/MS)

Analyze the products of enzymatic digestion, obtaining high-quality mass spectrometry data.

4. Data Processing and Full Sequence Assembly

Utilize advanced bioinformatics tools to analyze MS data and assemble the complete protein sequence.

 

Service Advantages

1. Completeness and Accuracy: The multi-enzyme system achieves nearly 100% peptide coverage, providing a comprehensive view of the protein sequence.

2. High Sensitivity: Effective analysis is possible even with trace amounts of sample, ensuring data reliability.

3. Detailed Analysis Report: Includes a thorough description of the experimental process and result interpretation, featuring protein sequence maps and analytical explanations.

 

Sample Submission Requirements

1. Sample Types

We accept various sample forms, including gel bands, gel spots, and liquid samples.

2. Sample Quantity

We recommend 10-20μg of protein.

3. Sample Purity

High-purity samples yield more reliable outcomes.

 

Applications:

1. Biopharmaceuticals: Determine the full-length sequences of biological agents like proteins, peptides, antibodies, and vaccines.

2. Functional Research: Explore protein functions and structures through comprehensive sequence analysis.

3. Post-translational Modification Analysis: Precisely identify protein post-translational modifications, offering essential insights for disease research and pharmaceutical development.

 

At MtoZ Biolabs, we not only provide top-quality protein full-length sequencing services but also tailor our solutions to meet specific client needs. Our expert team is ready to engage deeply with your projects to ensure precise and comprehensive support for your research. We look forward to collaborating with you to further scientific advancements.