Resources

Proteomics Databases



Metabolomics Databases

• • In-Depth Guide to CD: Techniques, Steps, and Data Analysis

  Circular dichroism spectroscopy (CD) is a spectroscopic technique commonly used to study the secondary structure of proteins and other biomolecules, such as α-helices, β-sheets, and random coils. CD spectra provide important information about molecular conformation and changes, particularly under varying external conditions like temperature, pH, and solvent environment.

• • Amino Acid Sequence Analysis Guide: Basics, Methods, and Practice

  The amino acid sequence refers to the arrangement of amino acids in a protein. This sequence is determined by genetic information, specifically through the process of transcription and translation of genes in DNA. Understanding amino acid sequences is important in biological research and fields like medicine, as they determine the structure and function of proteins.

• • Mechanisms of SEC and RPLC in Protein Purity Analysis

  In contemporary biochemistry and molecular biology research, protein purity analysis is indispensable. To guarantee the accuracy of subsequent experiments, such as structural determination, functional assays, or drug development, it is imperative to assess the purity of protein samples comprehensively.

• • Mechanism of HPLC-Based Protein Purity Detection

  Proteins are central molecules in life science research, and their purity directly impacts the reliability and reproducibility of experimental results. To ensure the purity of protein samples used in experiments, scientists have developed various analytical methods. Among these, High-Performance Liquid Chromatography (HPLC) has emerged as a crucial tool for protein purity detection, owing to its high resolution, sensitivity, and rapid analysis capabilities.

• • Applications of SEC and RPLC in Protein Purity Analysis

  In modern biological research, analyzing protein purity is a critical step for understanding protein functions and their roles in biological systems. High-Performance Liquid Chromatography (HPLC) techniques—namely Size Exclusion Chromatography (SEC) and Reversed-Phase Liquid Chromatography (RPLC)—are extensively used for this purpose.

• • Workflow of SEC and RPLC in Protein Purity Analysis

  Protein purity analysis is a critical step in biological research, enabling researchers to accurately assess the purity of protein samples, thereby ensuring the reliability of subsequent experiments. Two commonly used methods for protein purity analysis are Size-Exclusion Chromatography (SEC) and Reversed-Phase Liquid Chromatography (RPLC).

• • Advantages and Disadvantages of SEC and RPLC in Protein Purity Analysis

  Size Exclusion Chromatography (SEC) and Reversed-Phase Liquid Chromatography (RPLC) are two widely used techniques for protein purity analysis. Each has unique strengths and limitations, making them valuable in different research contexts.

• • Principle of SEC and RPLC in Protein Purity Analysis

  Protein purity analysis is crucial in biological research, where accurate assessment of protein sample purity is essential for subsequent experiments such as structural analysis, bioactivity testing, and drug development. Among the various protein purity analysis methods, Size Exclusion Chromatography (SEC) and Reversed-Phase Liquid Chromatography (RPLC) are widely adopted in both research and industry due to their high resolution and reliability.

• • Mechanism of SDS-PAGE in Protein Purity Analysis

  In the realm of proteomics, accurate and reliable analysis of protein purity is essential for numerous applications, ranging from drug development to clinical diagnostics. One of the cornerstone techniques in this domain is SDS-PAGE (Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis), a method renowned for its ability to separate proteins based on their molecular weight.

• • Application of SDS-PAGE in Protein Characterization

  In the rapidly advancing field of proteomics, precise protein characterization is critical for understanding biological processes and developing therapeutic interventions. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) stands out as a foundational technique, offering reliable analysis of protein purity, molecular weight, and homogeneity.