12,13-DiHOME Analysis Service
- Qualitative and quantitative analysis of 12,13-DiHOME
- Investigation of linoleic acid metabolism
- Potential application in biomedical studies
- Animal tissue ≥ 100 mg
- Plant tissue ≥ 200 mg
- Serum/ Plasma ≥ 200 μL
- Urine ≥ 2 mL
12,13-DiHOME is a hydroxylated derivative of linoleic acid, characterized by two hydroxyl groups at the 12th and 13th carbon positions of the octadecenoic acid chain. The molecule features a cis configuration (Z) at the 9th double bond. Its molecular formula is C18H34O4, and its molecular weight is 314.46 g/mol. Due to its amphipathic nature, 12,13-DiHOME exhibits both hydrophobic and hydrophilic interactions, which play a crucial role in its biological activity. Elevated levels of 12,13-DiHOME are associated with certain pathological conditions, including acute respiratory distress syndrome (ARDS) and cardiovascular diseases. Its concentration in the blood often increases in response to tissue injury or stress, indicating its potential as a biomarker for oxidative stress and inflammation. Additionally, the modulation of 12,13-DiHOME levels is being explored as a therapeutic target for metabolic diseases, such as obesity and diabetes, where its effects on lipid metabolism and insulin sensitivity could provide beneficial outcomes.
The quantification of 12,13-DiHOME in biological samples is typically performed using mass spectrometry-based techniques, such as liquid chromatography-tandem mass spectrometry (LC-MS/MS). These methods offer high sensitivity and specificity, enabling the detection and quantification of 12,13-DiHOME at low concentrations. This analysis is crucial for understanding its physiological and pathological roles, monitoring its levels in various diseases, and evaluating the effects of potential therapeutic interventions.
Figure 1. The Structure of 12,13-DiHOME
At MtoZ Biolabs, we utilize the latest liquid chromatography-mass spectrometry (LC-MS) platforms for the analysis of 12,13-DiHOME, a metabolite of linoleic acid metabolism with potential physiological relevance and biomedical interest.
Analysis Workflow
Service Advantages
1. Advanced Technological Platform
We use industry-leading mass spectrometry equipment and analysis techniques to ensure high precision and reliability of the analysis results.
2. Extensive Experimental Experience
With years of experience in experimental operations and data processing, we can handle various complex biological samples.
3. Rapid Data Delivery
Ensure quick sample processing and timely delivery of research results to facilitate scientific progress.
Applications
Sample Submission Requirements
1. Sample Types
Serum, plasma, urine, tissues and other biological samples.
Each sample should have at least 3 replicates under the same condition.
2. Sample Volume
3. Sample Preservation
Samples should be stored at -80°C to maintain stability.
Note: Detailed instructions on sample collection and handling will be provided.
Deliverables
1. Experimental Procedures
2. Relevant Liquid Chromatography and Mass Spectrometry Parameters
3. Detailed Information on 12,13-DiHOME
4. Raw Data
5. Custom Analysis Report
With our 12,13-DiHOME analysis service, we strive to support and advance your research by providing reliable, accurate, and comprehensive analysis.
How to order?
