4D-DIA Proteomics Analysis

Traditional proteomics technologies such as iTRAQ, TMT, and Label-free are all based on DDA (Data Dependent Acquisition) mode. In DDA mode, typically only the top 10-20 parent ions of highest abundance in the first-level mass spectrometry are selected for fragmentation, and their secondary mass spectrometry is printed for qualitative analysis. Since the DDA mode does not collect information on all ions, some proteins with lower abundance are often ignored, which to some extent limits our comprehensive understanding of the proteome. In contrast, DIA (Data Independent Acquisition) mode detects and fragments all ions to ensure that all ion information in the sample is obtained without omission and without difference.

 

4D-DIA proteomics is an emerging high-throughput mass spectrometry technology. It is based on the 4D-proteomics platform of timsTOF Pro2, introduces the dimension of ion mobility on the basis of DIA, not only significantly improves the accuracy and repeatability of quantitative analysis, but also achieves high stability and high precision quantitative analysis of proteomics in large sample queues.

 

Application Scenarios

1. Basic Biological Research

Reveal the molecular mechanisms of biological processes such as cell growth, differentiation, apoptosis, etc., by comparing the changes in proteomics under different conditions.

 

2. Disease Biomarker Screening

Screen proteins related to specific diseases by quantitative analysis of protein expression levels in patient samples, providing reliable indicators for early diagnosis, disease monitoring, and prognosis evaluation of diseases.

 

3. Drug Research and Evaluation

Reveal the mechanism of drug action by analyzing the impact of drugs on the proteome, providing important clues for new drug discovery and drug optimization.

 

4. Tumor Disease Molecular Typing

Screen potential tumor markers by comparing the protein expression differences between normal tissue and tumor tissue, providing strong support for early diagnosis, typing and treatment of tumors.

 

MtoZ Biolabs provides 4D-DIA proteomics research services with high sensitivity, detection depth and coverage based on the new generation of tims TOF Pro, assisting in research work such as trace sample proteomics, large sample population medicine and high-throughput modification proteomics research, free consultation is welcome.