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    5-methoxycarbonylmethyl-2-thiouridine Analysis Service

      5-methoxycarbonylmethyl-2-thiouridine (mcm⁵s²U) is a modified nucleoside found in tRNAs and rRNAs from bacteria and eukaryotes. It is derived from uridine, a component of RNA, through chemical modifications involving methylation and thiolation. These modifications are critical for the proper functioning of RNA molecules, particularly in protein synthesis. Studies have shown that mcm⁵s²U plays a significant role in the decoding center of the ribosome, thus affecting the accuracy of protein translation.

       

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      Figure 1. The Structure of 5-methoxycarbonylmethyl-2-thiouridine

       

      Understanding the roles of mcm⁵s²U in RNA biology can reveal crucial insights into the intricate mechanisms of RNA processing and protein synthesis. Moreover, the detection and quantification of mcm⁵s²U can become a potential biomarker for certain types of cancers and other diseases, making it a focus of pharmaceutical research.

       

      At MtoZ Biolabs, we offer a comprehensive mcm⁵s²U analysis service backed by our advanced LC-MS/MS systems and analytical expertise. Our team has extensive experience in metabolomics studies and is equipped to help our clients better understand the roles of mcm⁵s²U in their research.

       

      Analysis Workflow

       

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      Service Advantages

      • State-of-the-art LC-MS/MS platforms allow us to accurately and reliably quantify mcm⁵s²U in various types of samples.
      • A team of experts in mass spectrometry and metabolomics provide professional data analysis and interpretation.
      • One-stop service from sample preparation, LC-MS/MS analysis to data analysis, saving your time and efforts.
      • Competitive price with high-quality service.

       

      Applications

      Our mcm⁵s²U analysis service can be applied in various research fields such as:

      • Biomarker discovery
      • Disease diagnosis and prognosis
      • Drug response prediction
      • Basic biological research

       

      Sample Submission Requirements

      1. Sample Types

      Serum, plasma, urine, tissues, and other biological samples. It is recommended to prepare at least 3 biological replicates for each sample type.

       

      2. Sample Volume

      • Animal tissue ≥ 100 mg
      • Plant tissue ≥ 200 mg
      • Serum/ Plasma ≥ 200 μL
      • Urine ≥ 2 mL

       

      3. Storage and Shipping

      Samples should be stored at -80℃ and shipped with dry ice. Avoid repeated cycles of freezing and thawing

       

      Note: Please provide details on sample collection and handling.

       

      Deliverables

      1. Experimental Procedures

      2. Relevant Liquid Chromatography and Mass Spectrometry Parameters

      3. Detailed Information on 5-methoxycarbonylmethyl-2-thiouridine

      4. Raw Data

      5. Custom Analysis Report

       

      Contact our experts at MtoZ Biolabs to learn more about how our 5-methoxycarbonylmethyl-2-thiouridine analysis service can benefit your research.

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