CE-SDS Based Molecular Size Variation Analysis Service

Aggregation or fragmentation of protein molecules primarily causes variations in molecular size, impacting their biological activity, stability, and their efficacy and safety as pharmaceuticals. In the development and production of biopharmaceuticals, even slight variations in molecular size can reduce drug efficacy or pose safety risks. Thus, precise detection and analysis of these variations are crucial for bioproduct characterization. Capillary electrophoresis-sodium dodecyl sulfate (CE-SDS) uses an electric field to drive charged particles through a capillary, facilitating the separation and identification of proteins or peptides. By incorporating SDS, a surfactant, proteins acquire a negative charge and can be sorted by molecular size. CE-SDS combines the separation efficiency of electrophoresis with the precise measurement capabilities of SDS-PAGE, suitable for analyzing both individual and complex protein mixtures.

 



Figure 1. Analysis of Molecular Size Variability via CE-SDS

 

The CE-SDS-based analysis of molecular size variability is noted for its high resolution, sensitivity, and automation. This technique is extensively applied in the assessment of protein purity, molecular size determination, aggregation or fragmentation detection, disease diagnostics, and biopharmaceutical applications. It is of great importance in new drug development, protein function research, and biopharmaceutical quality control. MtoZ Biolabs has developed an integrated physicochemical analysis and structural characterization platform that facilitates the separation and comprehensive characterization of molecular size variants. Whether you are interested in protein fragments, conjugates, or aggregates, MtoZ Biolabs offers accurate, efficient, and all-encompassing solutions. Free consultations are available for further information.

 

Deliverables

In the technical report, MtoZ Biolabs will provide you with detailed technical information, including:

1. Experimental Procedures

2. Relevant Experimental Parameters

3. Detailed Information on CE-SDS-Based Molecular Size Variability Analysis

4. Electrophoresis Images

5. Raw Data