Analysis of Protein Vaccine Quality Peptide Map

Recombinant protein vaccines are created through biological engineering techniques, which involves inserting a part of the coding information (DNA or RNA) of a certain pathogen (such as a virus or bacteria) into a host cell. The host cell then produces a protein from the pathogen, which is then extracted and purified to make a vaccine. Mass peptide map analysis is a mass spectrometry analysis performed on these recombinant proteins to verify their quality and structure.

 

First, we need to understand what a mass spectrum is. A mass spectrum is a tool that characterizes the mass and structure of molecules. Through the mass spectrum, we can see the mass, structure and relative quantity of molecular substances. Each peak represents a different molecular substance, the height of the peak represents the relative quantity of the molecular substance, and the position of the peak represents the mass of the molecular substance. Thus, through the mass spectrum, we can perform accurate measurement and analysis of the protein's mass and structure. A mass peptide map, or mass spectrum, more specifically, refers to the mass spectrum used to depict peptide segments (small fragments after the protein has been enzymatically digested) during the mass spectrometry process. In this case, we are mainly concerned with the mass of the peptides and their interrelationships. With mass peptide map analysis, we can identify the composition, primary structure, and post-translational modifications of the recombinant protein, etc.

 

MtoZ Biolabs provides customers with drug quality research services that comply with global pharmaceutical regulations. Our recombinant protein vaccine mass peptide map analysis technology based on high-resolution chromatography and mass spectrometry platforms can effectively separate each peptide in complex samples. Mass spectrometry can also fragment peptides to generate a series of peptide ion fragments, thus obtaining peptide sequence information. This is very important for the identification, quantification, and functional research of recombinant proteins. It can be used to confirm the integrity, structure, and modification of recombinant proteins, providing key information for the development and clinical application of recombinant proteins. At the same time, we also provide customized technical services to meet the needs of different customers. Free consultation is welcome.