Antibody Sequencing and Immunome Sequencing
Antibody sequencing is a technique that analyzes the amino acid sequences of antibodies (immunoglobulins), which helps researchers understand the specificity of antibody-antigen interactions. Immunosequencing, on the other hand, examines DNA sequences associated with the immune system, aiding researchers in understanding immune responses to various diseases and infections. These insights enable the design and development of more effective vaccines and antibody therapies.
The Process of Antibody Sequencing Typically Includes the Following Steps
1. mRNA is extracted from B cells.
2. Reverse transcriptase, an enzyme, is used to transcribe mRNA into cDNA.
3. The cDNA is then amplified by PCR to generate numerous copies of antibody genes.
4. These genes are sequenced, and the results are compared to known antibody databases to determine the antibodies’ amino acid sequences.
The Process of Immunosequencing Generally Involves These Steps
1. Whole genome DNA is extracted from immune cells.
2. This DNA is sequenced to reveal its complete nucleotide composition.
3. Bioinformatics tools are employed to compare these sequences with a reference genome, identifying the locations and functions of immune-related genes.
Both antibody sequencing and immunosequencing are critical in elucidating immune system functions. When combined, these techniques offer a comprehensive view of immune responses, thus facilitating the development of novel immunotherapies and vaccines.
MtoZ Biolabs, an integrated chromatography and mass spectrometry (MS) services provider.
Related Services
How to order?
