Autofluorescence Spectral Analysis of Recombinant Protein Drugs

Recombinant protein drugs refer to protein products that originate from animals or plants and are developed through biotechnological research. These products have specific biological activity and can prevent, treat, and diagnose diseases in humans, animals, and plants. Compared with small molecule drugs, recombinant protein drugs have advantages such as high activity, high specificity, and low toxicity, making them favored by many researchers. At present, recombinant protein drugs have been widely used in various fields such as tumors, autoimmune diseases, metabolic diseases, aging diseases, and degenerative diseases. Understanding the biochemical properties and 3D structure of recombinant protein drugs is crucial as their activity, stability, toxicity, efficacy, and shelf life can be affected by structure-activity relationships.

 

Fluorescence spectroscopy is an important research tool for studying the real-time structure and dynamics of proteins/peptides in solution and under microscopy. Spontaneous fluorescence spectroscopy refers to the spontaneous emission of fluorescence by proteins without an external excitation light source. The properties and structure of substances are studied by measuring the intensity and wavelength distribution of fluorescence. Through spontaneous fluorescence spectroscopy analysis, we can obtain important information about the structure and environment of recombinant protein drugs.

 

MtoZ Biolabs provides drug quality research services that comply with global pharmaceutical regulations. We offer one-stop spontaneous fluorescence spectroscopy analysis service for recombinant protein drugs. This technology can detect structural changes or the binding of two molecules by changing the intensity of fluorescence; it can also locate tryptophan residues (either on the protein surface or deeply buried within the protein) through the wavelength of tryptophan fluorescence; additionally, it can study the migration rate of fluorescent groups through fluorescence polarization and anisotropy.