Capillary Electrophoresis Analysis

Capillary electrophoresis (Capillary Electrophoresis, CE) is a separation technique which makes use of the difference of the migration velocity of molecules or ions in liquid medium in the capillary under the action of electric field. This method is characterized by high efficiency, high resolution, and rapid analysis, and is widely used in biochemistry, molecular biology, drug analysis, and other fields.

 

Capillary electrophoresis is based on the principle of different migration speeds of molecules or ions in an electric field. The migration speed of molecules in an electric field depends on their charge, size, and shape, as well as the viscosity of the medium and the strength of the electric field.

 

Advantages

1. High Efficiency and High Resolution

Due to the small diameter of the capillary, the temperature can be effectively controlled, reducing the influence of heat on the analysis results, thereby providing high-efficiency and high-resolution separation.

 

2. Low Sample Consumption

Capillary electrophoresis requires only a very small amount of sample and reagents, making it suitable for micro-sample analysis.

 

3. Wide Application

Capillary electrophoresis is applicable to various types of samples, including proteins, nucleic acids, small molecule drugs, and complex biological samples.

 

4. Various Modes

Including Capillary Zone Electrophoresis (CZE), Capillary Gel Electrophoresis (CGE), Capillary Isoelectric Focusing (CIEF), and Capillary Isotachophoresis (CITP), etc.

 

5. Flexibility

By changing the composition of the electrophoresis buffer, the type and size of the capillary, it can be optimized for different samples and applications.

 

Due to the characteristics mentioned above, capillary electrophoresis has become a very important tool in modern analytical chemistry and biochemistry research.