Chemistry Proteomics

Chemical proteomics is one of the important means of research in chemical biology. Different from previous proteomics techniques that qualitatively or quantitatively identify proteins, chemical proteomics aims to solve the problem of how proteins in complex systems such as cell lysates, live cells, and tissues interact with small molecule drugs through a series of chemically diverse probes in combination with proteomics technology. It has been widely used in research such as small molecule drug target discovery and screening of drug lead compounds.

 

The general process of chemical proteomics is to incubate chemical probes with affinity matrices or reporter groups with the proteome, use affinity purification methods to enrich proteins bound to the probe, and then analyze them with other auxiliary technologies such as mass spectrometry. There are two commonly used methods for small molecule drug target research based on chemical proteomics, which are Activity-based protein profiling (ABPP) based on active probes and Compound-centric chemical proteomics (CCCP).

 

ABPP

The principle of ABPP is to modify active drug molecules to synthesize probes, these probes can bind to target proteins and affect their activity. ABPP synthesizes probes with reactive groups and markers, and these probes interact with proteins, thereby achieving the study of protein function. The advantage of ABPP is that it labels the active form of the enzyme, not the inactive form, so it is particularly suitable for analyzing enzyme activity in different cell types and tissues, and for detecting differences between normal and disease states. In addition, it is often used for drug target screening.

 

CCCP

The principle of CCCP is to modify drug molecules and introduce labeled molecules, which are then fixed to the matrix (such as agarose beads or other resins), enrich proteins that can bind to drug molecules, and then identified by mass spectrometry. Compared with ABPP, CCCP does not need to provide direct information about the active state of the protein, and as a more non-directional method, CCCP can identify unexpected biomolecules, including proteins without enzyme function, so it can be used to explore completely unknown novel drug targets. At the same time, CCCP has high-throughput characteristics and can simultaneously analyze the interaction of multiple compounds with proteins in cell extracts or live cells.

 

MtoZ Biolabs' comprehensive solution for chemical proteomics includes the whole process from experimental design, sample preparation, probe synthesis, target identification and data analysis, providing you with a one-stop worry-free service, meeting your various needs in biomarker research, drug and target discovery, pathway modeling, drug action research and so on in the field of drug development and drug discovery. With our comprehensive solution, you will not waste time and cost on the wrong candidate drugs, and can optimize the success rate of drug development and drug discovery programs. Feel free to consult and learn more about the details of the service!