Cis(+)-12-Oxophytodienoic Acid Analysis Service
- Accurate quantification of OPDA using high-resolution mass spectrometry.
- Comprehensive data analysis including metabolic pathway analysis.
- A dedicated team of experts in mass spectrometry and metabolomics to assist with data interpretation.
- Confidential and reliable service.
- Plant biology research
- Agricultural research
- Plant breeding and genetics
- Botanical drug research
- Quantity: At least 200 μl liquid or 200 mg tissue.
- Storage and Shipping: Samples should be stored at -80℃ and shipped with dry ice.
- For each sample, it is necessary to provide a minimum of 3 replicates with the same conditions.
Cis(+)-12-Oxophytodienoic Acid (OPDA) is a crucial intermediate in the biosynthesis of jasmonic acid, a plant hormone that plays a significant role in plant defense mechanisms and developmental processes. Structurally, OPDA is a cyclopentenone derivative, formed through the oxidation of linolenic acid via the octadecanoid pathway. The formation of OPDA involves the action of several key enzymes, including lipoxygenase (LOX), allene oxide synthase (AOS), and allene oxide cyclase (AOC), which together convert linolenic acid into OPDA within the chloroplast.
Once synthesized, OPDA can act directly as a signaling molecule, influencing gene expression related to plant defense and growth. Additionally, it serves as a precursor for the production of jasmonic acid through subsequent reductions and β-oxidation steps in the peroxisome. The conversion of OPDA to jasmonic acid is tightly regulated and is crucial for the activation of plant responses to biotic and abiotic stresses, including herbivory, wounding, and pathogen attacks.
Research on OPDA is vital for understanding the intricate networks that regulate plant stress responses and development. By investigating the levels and activity of OPDA, scientists can gain insights into the plant's defense mechanisms and identify potential strategies for enhancing crop protection and productivity.
Figure 1. Biosynthetic Pathway Toward cis-(+)-12-OPDA (4) and Undesired Side Products
We understand that precise and accurate measurement of OPDA levels in plant samples is of paramount importance in plant biology research. At MtoZ Biolabs, we provide:
Analysis Workflow
Our OPDA analysis service involves the following steps:
1. Sample Preparation: Plant tissues are meticulously prepared to avoid any contamination that can affect the analysis.
2. Metabolite Extraction: Standard protocols are followed for the extraction of OPDA from the prepared samples.
3. Mass Spectrometry Analysis: The extract is analysed using cutting-edge mass spectrometry equipment to measure the OPDA levels.
4. Data Analysis: The data generated is rigorously analysed to provide meaningful insights into the OPDA content and its metabolic pathways.
5. Report Generation: A comprehensive report detailing the analysis results is prepared.
Application
Our cis(+)-12-oxophytodienoic acid analysis service is valuable in various fields such as:
Sample Submission Requirements
We accept a variety of plant tissue samples and other biological samples. However, for the best results, please ensure:
For specific sample submission requirements, please contact our customer service team.
Deliverables
1. Experimental Procedures
2. Liquid Chromatography and Mass Spectrometry Parameters
3. Detailed Information on Cis(+)-12-Oxophytodienoic Acid
4. Raw Data
5. Custom Analysis Report
Contact us today for more information about our cis(+)-12-oxophytodienoic acid analysis service. At MtoZ Biolabs, we are committed to providing top-notch analysis services to help you in your research.
How to order?
