Diacylglycerol Analysis Service

Diacylglycerol (DAG), also known as diglyceride, consists of two fatty acid chains and a glycerol molecule. It primarily exists in two forms: 1,2-diacylglycerol and 1,3-diacylglycerol. DAG can be synthesized via the phosphatidic acid pathway in the endoplasmic reticulum by the action of phosphatidic acid phosphatase or produced during the lipolysis of triglycerides (TAG) by TAG lipase. DAG is also a crucial substrate for the biosynthesis of triglycerides, which are essential for energy storage, and phospholipids such as phosphatidylcholine (lecithin) and phosphatidylethanolamine, which are both vital for cell membrane structure.

 

DAG acts as a second messenger involved in the prolonged activation of various signal transduction pathways. The stereochemistry of DAG isomers influences their specific roles in metabolic processes. Diacylglycerol kinases (DGKs) play a significant role in cell signaling. It can terminates the signaling function of DAG to ensuring DAG's stable and controlled availability by phosphorylating DAG. One of the primary targets of DAG is protein kinase C (PKC). It has shown that DAG can enhance the activity of PKC, which in turn activates downstream targets via serine and threonine phosphorylation. Moreover, DAG is pivotal in membrane dynamics, such as inducing membrane fusion through partial dehydration of the bilayer surface and the increased lipid monolayer curvature.

 

DAG has been identified in various biological processes. Intracellular abnormal accumulation of DAG is positively correlated with disruptions in insulin signaling. Additionally, studies on transgenic obese animals have demonstrated that insulin resistance is associated with increased DAG levels in liver and muscle tissues. DAG is also implicated in the pathophysiology of cancer, neural signaling, and immune regulation. It is important to study DAG since its diverse roles in biological processes.

 

The LC-MS platform in MtoZ Biolabs can detect a wide range of DAG species, providing valuable data for studying relative changes in DAG concentrations, mechanistic insights, and biomarker discovery. MtoZ Biolabs employs robust, reproducible, and sensitive systems for the separation, characterization, identification, and quantification of DAG, ensuring reliable, rapid, and cost-efficient analyses. Free project evaluation!

 

Services at MtoZ Biolabs

1. Dilinoleoyl-Glycerol

2. Palmitoyl-Linoleoyl-Glycerol

3. Dipalmitoyl-Glycerol

4. Palmitoyl-Oleoyl-Glycerol

5. Stearoyl-Linoleoyl-Glycerol

6. Dioleoyl-Glycerol

7. Stearoly-Oleoyl-Glycerol