Deciphering the Mysteries of Life Processes Using Protein Absolute Quantification Technology

The Importance and Principle of Protein Absolute Quantification Technology

1. The Importance of Protein Absolute Quantification Technology

Accurate measurement of the quantitative levels of proteins is a basic element of understanding biological systems, which helps to reveal the expression changes of proteins, the regulation of signaling pathways, and the functional regulation, etc.

 

2. The Principle of Protein Absolute Quantification Technology

This technology measures the absolute concentration of proteins in samples accurately by calibrating protein standards with known concentrations in the sample and combining with the signal intensity of mass spectrometry instruments. Commonly used methods include Multiple Reaction Monitoring (MRM), Isotope Labeling, and Internal Standard Method, etc.

 

Sample Preparation and Experimental Procedures of Protein Absolute Quantification Technology

1. Sample Preparation

This includes the extraction, washing, and purification of proteins to ensure the purity and integrity of the sample and reduce the influence of interferences.

 

2. Standard Curve and Internal Standard Substance

Establishing a standard curve and using an internal standard substance for calibration and correction of absolute quantitative analysis improves the accuracy and reliability of the results.

 

3. Mass Spectrometry Analysis and Data Processing

Samples are analyzed using a mass spectrometer to obtain the protein mass spectrometry signal, and professional software is used to process and analyze the data to obtain absolute quantitative results.

 

Application

1. Biological Process Analysis

Absolute quantification of different proteins in cells or tissues can reveal the dynamic expression and interaction of proteins in biological processes, such as cell cycle regulation, signal transduction pathways, and gene expression regulation, etc.

 

2. Disease Mechanism Research

By comparing the difference in absolute quantification of proteins in disease samples and normal samples, disease-related protein biomarkers can be discovered to deepen our understanding of the occurrence and development mechanisms of diseases.

 

3. Personalized Medicine and Drug Development

Protein absolute quantification technology can be used to evaluate drug target effects and drug metabolism, providing a critical basis for personalized medicine and drug development.

 

Protein absolute quantification analysis is an essential tool for deciphering life processes. It can accurately measure the quantitative levels of proteins and reveal the secrets of biological systems. Through the standard operation of sample preparation and experimental procedures, as well as the accurate operation of mass spectrometry analysis and data processing, protein absolute quantification analysis has a broad application prospect in life science research and medical progress.