Dehydrolithocholic Acid Analysis Service

Dehydrolithocholic acid (DLCA) is an oxidized metabolite derived from lithocholic acid, primarily found in bile. In the liver, lithocholic acid undergoes enzyme-mediated oxidation to form DLCA, a crucial step in the bile acid metabolic pathway. As an intermediate in this pathway, DLCA plays a significant role in the synthesis, detoxification, and excretion of bile acids. It is also essential in maintaining the enterohepatic circulation of bile acids and ensuring normal liver function. Specifically, DLCA aids in preventing bile acid-induced cellular damage by promoting the excretion of hydrophobic bile acids and reducing their cytotoxic effects. Fluctuations in DLCA levels are closely linked to various hepatobiliary diseases and metabolic disorders, such as cholestasis, cirrhosis, and hepatobiliary cancers. Therefore, the precise quantification of DLCA is vital not only for understanding its role in bile acid metabolism but also for early diagnosis of liver diseases, monitoring disease progression, and conducting drug metabolism research.

 

Figure 1. Molecular Structure of Dehydrolithocholic Acid

 

MtoZ Biolabs offers Dehydrolithocholic Acid Analysis Service using high performance liquid chromatography-mass spectrometry (HPLC-MS). This advanced method combines the separation capabilities of HPLC with the detection power of MS, ensuring high sensitivity and specificity in DLCA detection.

 

Analysis Workflow

 



 

Service Advantages

• Rapid Analysis Cycle and Cost-Effectiveness
• Stable, Reliable, and Highly Sensitive Results
• Comprehensive One-Stop Service for Extensive Testing Needs
• User-Friendly Operation with Wide Application Scope
• Supported by Extensive Bioanalytical Resources and Advanced Platforms

 

Sample Submission Requirements

1. Sample Types

Serum, plasma, urine, feces, tissue extracts, and other biological samples.

 

2. Sample Volume

At least 200 μL of liquid sample or 200 mg of solid sample.

 

3. Sample Preservation

Store at -80°C to maintain stability.

 

Note: Provide details on sample collection and handling.

 

Deliverables

1. Experimental Procedures

2. Relevant Liquid Chromatography and Mass Spectrometry Parameters

3. Detailed Information on Dehydrolithocholic Acid

4. Raw Data

5. Custom Analysis Report

 

Our Dehydrolithocholic Acid Analysis Service provides high-quality data support, advancing your scientific research and applications. For any inquiries or requirements, please contact us.