DIA Phosphoproteomics Analysis Service

Protein phosphorylation plays a critical role in regulating cell signaling, metabolism, proliferation, and apoptosis, and is integral to both physiological and pathological processes. Traditional data dependent acquisition (DDA) proteomics, which primarily captures high-abundance parent ions, often overlooks low-abundance phosphorylated peptides, thereby restricting the depth and accuracy of quantitative phosphoproteomic analysis. Data independent acquisition (DIA) phosphoproteomics technology overcomes these challenges by utilizing an unbiased full-spectrum scanning mode, capturing all ion data from a sample. This results in significant improvements in data coverage, reproducibility, and sensitivity.

 

At MtoZ Biolabs, our DIA Phosphoproteomics Analysis Service combines DIA technology with immobilized metal ion affinity chromatography (IMAC) for efficient enrichment and precise quantification of phosphorylated peptides. This approach ensures comprehensive detection of low-abundance phosphorylation events, providing deep coverage of phosphorylation sites. With a detection depth capable of identifying over 10,000 phosphorylation sites per sample, DIA is particularly suited for the high-throughput analysis of complex biological samples. By enabling detailed qualitative and quantitative analysis of phosphorylation modifications, researchers can delve deeper into the regulatory roles of phosphorylation in cellular signaling pathways and disease pathogenesis.

 

Analysis Workflow

 



 

Service Advantages

1. Full-Spectrum Scanning

The data-independent acquisition mode minimizes high-abundance ion interference and captures both high- and low-abundance signals. Compared to label-free proteomics, DIA improves data coverage by up to 40%.

 

2. High Reproducibility and Stability

DIA offers a 40% improvement in identification repeatability and nearly doubles quantification precision compared to conventional methods.

 

3. Superior Quantification Accuracy

DIA's quantitative accuracy approaches that of SRM/MRM targeted proteomics, considered the gold standard in precise quantification.

 

4. Large Cohort Traceability

With the ability to analyze large sample cohorts, DIA reduces batch effects and supports retrospective and integrated data analysis across multiple experiments.

 

Sample Submission Requirements

 



For more sample details, please consult our technical team.

 

Applications

DIA phosphoproteomics is widely applicable across biological research fields, particularly for studying dynamic phosphorylation changes in cell signaling and disease mechanisms. Key applications include:

1. Tumor and Neurological Disease Research

Comprehensive mapping of phosphorylation pathways to uncover molecular regulatory mechanisms in cancer and neurological disorders.

 

2. Drug Mechanism of Action and Target Screening

Ideal for experiments involving multiple concentrations and time points, enabling the identification of critical drug targets.

 

3. Tumor Classification Studies

Supports large-scale analyses for tumor classification and identification of disease-specific features.

 

4. Plant Hormone Regulation Research

Enables dynamic analysis of phosphorylation changes within plant hormone signaling pathways during growth and development.

 

MtoZ Biolabs' DIA Phosphoproteomics Service provides researchers with a robust, high-throughput solution for the in-depth analysis of phosphorylation modifications. Leveraging advanced DIA technology and enrichment techniques, combined with comprehensive data analysis tools, we help researchers uncover the vital roles of phosphorylation in cellular signaling, disease progression, and therapeutic development, driving forward the frontiers of scientific discovery. Free project evaluation, welcome to learn more details! Our technical specialists are available to provide a free business assessment.