Dia Proteomics

DIA (Data-Independent Acquisition Proteomics) is an advanced mass spectrometry technique used for in-depth proteomics analysis. Compared with traditional data-dependent acquisition (DDA) methods, DIA technology offers higher coverage and quantitative accuracy in proteomics research, allowing researchers to more comprehensively analyze the proteins in a sample.

 

The core of DIA proteomics lies in its method of mass spectrometry acquisition. In DIA mode, the mass spectrometer does not selectively analyze specific peptide segments based on precursor ion intensity, but systematically scans the entire mass range. This means that the mass spectrometer will collect spectral information for all peptide segments in the sample, not just the most abundant or significant ones. The main advantages of this approach include:

 

More Comprehensive Sample Coverage

DIA technology can analyze more peptide segments and proteins, providing a more comprehensive proteome coverage.

 

Higher Data Reproducibility

The data collected by DIA has higher reproducibility, which is especially important for quantitative proteomics.

 

Better Quantitative Accuracy

DIA methods provide more accurate protein quantitation information.

 

Stronger Dynamic Range

It can detect proteins of lower abundance, which is especially important for the discovery of biomarkers.

 

DIA proteomics has shown tremendous potential in fields such as clinical research, biomarker discovery, and disease mechanism research. With the continuous advancement of mass spectrometers and data analysis methods, the application range of DIA proteomics is expected to further expand.