Edman Sequencing Service
Edman sequencing has been widely used in protein chemistry and proteomics due to its high accuracy and ability to provide detailed insights into the primary structure of proteins. Edman sequencing directly sequences peptides by sequentially identifying each amino acid from the N-terminus, making it particularly useful for smaller peptides and proteins or in cases where mass spectrometry may not be optimal. Edman sequencing serve as a crucial tool for applications ranging from protein identification and post-translational modification analysis to confirming the structure of novel peptides and therapeutic proteins.
Edman sequencing operates on a straightforward yet highly effective chemical process, where the N-terminal amino acid of a peptide or protein is selectively cleaved and identified. The process of edman sequencing begins with the attachment of the phenylisothiocyanate (PITC) reagent to the free amino group at the N-terminus of the peptide. This reaction forms a cyclic derivative known as a phenylthiohydantoin (PTH) derivative, which is then cleaved from the peptide. The PTH-amino acid is subsequently analyzed using chromatographic or spectroscopic methods to identify the specific amino acid. The process is repeated in successive cycles, each time removing and identifying one amino acid from the N-terminus. With each cycle, the remaining peptide becomes shorter, and the sequence of amino acids can be read step by step, providing a complete and accurate representation of the protein’s primary structure.
Services at MtoZ Biolabs
At MtoZ Biolabs, we offer specialized Edman Sequencing services designed to meet the needs of researchers across various fields. Our advanced technology, paired with expert scientists, ensures highly accurate and reliable amino acid sequencing, even from small or challenging samples. Whether you're conducting protein structure analysis, identifying novel peptides, or confirming protein sequences, our Edman Sequencing service provides the precision and sensitivity you need.
We tailor our services to your specific project requirements, delivering customized solutions that optimize the sequencing process for diverse applications, such as studying protein-protein interactions, characterizing post-translational modifications, or analyzing complex protein mixtures. With state-of-the-art equipment, rigorous quality control, and fast turnaround times, we ensure that you receive the most accurate and timely results.
Figure 1. The General Workflow of HPLC–MS/CD-Based Detection of Liberated N-Terminus Phenylthiohydantoin Amino Acids Method
Why Choose MtoZ Biolabs?
1. MtoZ Biolabs established an advanced Edman Sequencing Service platform, guaranteeing reliable, fast, and highly accurate analysis service.
2. Deep data coverage with strict data quality control. AI-powered bioinformatics platform integrates all edman sequencing data, providing clients with a comprehensive data report.
3. Accurately distinguishes similar amino acids, such as I/L and Q/K.
4. Our pricing is transparent, no hidden fees or additional costs.
5. The process from sample handling to report generation is efficient, shortening the analysis cycle.
6. Tailored service to suit specific research needs.
Applications
1. Edman Sequencing is ideal for determining the N-terminal sequence of proteins, peptides, antibodies, and vaccine candidates, ensuring precise identification and characterization.
2. Edman Sequencing is used to verify the correct translation of recombinant proteins, ensuring that the expressed proteins match the intended sequence and structure.
3. Edman Sequencing confirms the sequence of synthesized peptides, ensuring high-quality and accurate peptide synthesis for research or therapeutic applications.
Case Study
CD Spectrum of the PTH Derivative of BrPhe and Ion Chromatograms of DLA Derivatives
Hahn D. et al. Sci Rep. 2022.
FAQ
Q: What types of samples are not suitable for Edman Sequencing analysis?
Edman Sequencing is not suitable for samples lacking a free N-terminus, such as proteins or peptides with blocked or modified N-terminal groups. Additionally, if the targeted N-terminal sequence contains a high proportion of non-standard amino acids without available reference standards, sequencing becomes unreliable. In these cases, alternative techniques such as mass spectrometry can be used for sequence confirmation and to overcome these limitations.
Deliverables
1. Comprehensive Experimental details
2. Materials, Instruments, and Methods
3. Total Ion Chromatogram & Quality Control Assessment
4. Data Analysis, Preprocessing, and Estimation
5. Bioinformatics Analysis
6. Raw Data Files
MtoZ Biolabs, an integrated Chromatography and Mass Spectrometry (MS) Services Provider, provides advanced proteomics, metabolomics, and biopharmaceutical analysis services to researchers in biochemistry, biotechnology, and biopharmaceutical fields. Our ultimate aim is to provide more rapid, high-throughput, and cost-effective analysis, with exceptional data quality and minimal sample consumption. If you are interested in our Edman Sequencing Service, please feel free to contact us.
How to order?
