ESI-MS/MS Analysis Service

Electrospray Ionization Tandem Mass Spectrometry (ESI-MS/MS) is a mass spectrometry analytical method that combines electrospray ionization (ESI) with tandem mass spectrometry (MS/MS). ESI is a technique that uses an electric field to convert a sample solution into charged mist-like droplets, thereby achieving ionization of the sample. Tandem mass spectrometry involves introducing a second stage of mass analysis after the first, analyzing fragment ions produced from precursor ions to obtain structural and compositional information for subsequent data analysis.

 

The ESI-MS/MS analysis service integrates the high ionization efficiency of electrospray ionization with the high resolution and selectivity of tandem mass spectrometry. It allows for the efficient and accurate analysis of complex samples, particularly those that are highly polar, non-volatile, or thermally unstable. This method provides precise and sensitive molecular mass and structural information. In recent years, LC-MS instruments based on the ESI-MS/MS principle have become indispensable tools in scientific research.

 

MtoZ Biolabs leverages the high efficiency, speed, and accuracy of the ESI-MS/MS analysis service system to establish robust technical capabilities in proteomics, peptidomics, metabolomics, modification analysis, characterization, and protein sequencing.

 



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Figure 1. Schematic diagram of ESI-MS/MS.

 

Service Advantages

Leveraging ultra-high-performance liquid chromatography (UHPLC) and high-resolution mass spectrometry (HRMS), MtoZ Biolabs’ ESI-MS/MS analysis service provides comprehensive detection capabilities for macromolecules (proteins, peptides, metabolites) and small molecules. With the high resolution and high throughput of ESI-MS/MS, our service offers two core advantages:

 

1. Extensive Instrument Support

ESI-MS/MS has become an indispensable tool in scientific research, with increasing demands on instrumentation. To meet these requirements, MtoZ Biolabs integrates a wide range of state-of-the-art instruments from multiple manufacturers, including but not limited to:Vanquish Neo-Orbitrap Exploris 480,Vanquish Neo-Orbitrap Fusion Lumos,Vanquish Neo-Orbitrap Astral,IMS TOF Pro2. These instruments cooperate with each other to achieve the identification and relative quantification of proteins and metabolites (3D & 4D) at the omics level, the sequencing and analysis of proteins at the amino acid level, and modified omics such as protein phosphorylation, lactylation, and ubiquitination, and characterization-level detection, as well as absolute quantitative analysis of specific proteins.

 



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Figure 2. Orbitrap Fusion Lumos three-in-one mass spectrometer

 

2. Customized Acquisition Strategies

MtoZ Biolabs offers ESI-MS/MS analysis service tailored to meet diverse client needs with customized liquid-phase separation and mass spectrometry acquisition protocols. In the aspect of sequencing, several optimized acquisition schemes are adopted, and Vanquish Neo LC system and Orbitrap Fusion Lumos three-in-one high-resolution MS cooperate with each other to realize the accurate judgment of I and L in the protein, so as to realize the accurate judgment of every amino acid sequence of any protein/antibody.For omics-level analysis, we utilize the cutting-edge Orbitrap Astral mass spectrometer, leveraging its high throughput and resolution to perform rapid mass spectrometry analysis on large sample sets. Compared to traditional mass spectrometry methods, our approach not only saves time but also ensures precise control over sample analysis.

 

By combining ESI-MS/MS technology combined with various instruments and accurate angle analysis, our business can cover multiple levels and provide fast and accurate detection for different detection requirements provided by customers.

 

Sample Submission Suggestions

1. Sample Types

MtoZ Biolabs accepts a variety of sample types, including gel bands, protein solutions, solid powders, cells, animal and plant tissues, metabolites and so on.

 

2. Sample Amounts

According to the sample type and the project has the slight difference, the concrete may consult us.

*Note: For any special requirements or assistance with sample preparation, please contact us.

 

Applications

ESI-MS/MS analysis service provide the foundation for a wide range of applications:

 

1. Characterization Analysis

Protein drug N-terminal/C-terminal sequence analysis;Peptide mapping of protein drugs;Glycosylation analysis of protein drugs;Disulfide bond analysis of protein products,ect.

 

2. Immunology Analysis

Identification of immunogenic fragments in protein drugs by analyzing MHC-I and MHC-II antigen-presenting peptides.

 

3. Protein Sequencing Analysis

With ESI-MS/MS and the ETD module in the Orbitrap Fusion Lumos, we can accurately distinguish Leucine (L) from Isoleucine (I) and derive precise protein sequences.

 

4. Proteomics and Metabolomics Analysis

Efficient enrichment of specific modified peptides and Identification and relative quantification of proteins and metabolites at the omics level

 

Case Study

ESI-MS/MS analysis service is a basic life science research method, which can be extended to more application scenarios, to compare chondrosarcomas before and after cryoablation, and to look for clues that might be useful in the diagnosis and Pathophysiology of chondrosarcomas, leading to the development of new and effective treatments for sarcomas. In order to find differential proteins in the two groups, ESI-MS/MS was used to analyze the samples before and after treatment. Finally, more than 160 kinds of total differential proteins were identified. Most of the identified proteins are associated with various important pathways such as glycolysis, MAPK activation, PI3K-Akt signaling, extracellular matrix degradation, etc. This study illustrates the great significance of cryoablation treatment for chondrosarcoma, and it is the first to investigate the chondrosarcoma protein difference before and after treatment in the form of protein profile, which can be used as a basis for future research, especially in the treatment of chondrosarcoma, to find a new way for the treatment of chondrosarcoma.

 



 

Rashmi Madda, E. et al. J. Am. Soc. Mass Spectrom. 2021.

Figure 3. Effect of cryoablation treatment on chondrosarcoma

 

Deliverables

1. Comprehensive Experimental Details

 

2. Materials, Instruments, and Methods

 

3. Total Ion Chromatogram & Quality Control Assessment (project-dependent)

 

4. Data Analysis, Preprocessing, and Estimation (project-dependent)

 

5. Bioinformatics Analysis

 

6. Raw Data Files

 

MtoZ Biolabs, an integrated Chromatography and Mass Spectrometry (MS) Services Provider, provides advanced proteomics, metabolomics, and biopharmaceutical analysis services to researchers in biochemistry, biotechnology, and biopharmaceutical fields. Our ultimate aim is to provide more rapid, high-throughput, and cost-effective analysis, with exceptional data quality and minimal sample consumption. Free project evaluation, welcome to learn more details!