Exosome Proteomics Service

Exosome proteomics is an efficient tool for studying the composition, structure, and function of proteins within exosomes. Exosomes are nanoscale vesicles secreted by cells, widely present in various biological fluids, and play a crucial role in intercellular communication. Through proteomic technologies, researchers can comprehensively analyze the types and relative abundances of proteins within exosomes, revealing their specific roles in biological processes. Exosomes carry a wealth of biomarkers, which are of significant value in disease diagnosis, prognosis assessment, and the discovery of therapeutic targets. Particularly in complex diseases such as cancer, neurodegenerative disorders, and cardiovascular diseases, exosome proteomics provides key molecular information, helping scientists gain a deeper understanding of disease mechanisms and progression. Exosome proteomics can address key issues across multiple fields. Firstly, it demonstrates enormous potential in clinical diagnostics by identifying specific exosomal protein biomarkers, enabling early disease detection and precise diagnosis. Secondly, in drug development, exosome proteomics aids in discovering new therapeutic targets and biomarkers, accelerating the development and optimization of new drugs. Additionally, the advancement of personalized medicine also benefits from exosome proteomics, as analyzing patient-specific exosomal protein profiles allows for the formulation of more personalized and effective treatment plans. Lastly, in basic research, exosome proteomics plays an important role in helping scientists uncover the complex mechanisms of intercellular communication and their changes under physiological and pathological conditions. Therefore, this field not only broadens the horizons of biomedical research but also provides rich data support and theoretical foundations for clinical applications.

 

MtoZ Biolabs provides professional and comprehensive exosome proteomics analysis services, capable of identifying and quantifying proteins in exosomes. By integrating bioinformatics techniques for the analysis of proteomic data, the detection of biomarkers can be achieved. Trust MtoZ Biolabs for precise and comprehensive exosome proteomics analysis.

 



Sun, Y. et al.  Anal. Chim. Acta. 2017.

Figure 1. Saliva and Serum Exosome Proteomics Workflow

 

Service Advantages

1. One-stop Exosome Proteomics Analysis

We can complete the entire process including exosome separation to protein identification and quantification. It is possible to separate exosomes from various types of samples, including plasma/serum, cerebrospinal fluid, saliva, snot, urine, bile, and cell culture media.

 

2. Advanced Facilities and Optimized Operation Procedures

MtoZ Biolabs uses the currently highest resolution and sensitivity mass spectrometer, the newly introduced Obitrap Fusion Lumos by Thermo company, combined with nanoLC chromatography for exosomal proteomics analysis. MtoZ Biolabs has extensive experience in the field of exosome proteomics analysis and can provide customized services.

 

3. Biomarker Detection through Bioinformatics Analysis

Our professional bioinformatics analysts are proficient in using various bioinformatics analysis tools, providing in-depth analysis and data mining.

 

Case Study

1.Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the Highest Abundant Protein and a Putative Universal Biomarker

Exosomes are extracellular vesicles derived from endosomal compartments and may participate in intercellular communication. In this study, researchers found that commonly used biomarkers for exosomes are heterogeneous and do not exhibit universal utility across different cell types. To identify universally abundant proteins, they employed an unbiased quantitative exosome proteomics approach based on super-SILAC (super Stable Isotope Labeling by Amino acids in Cell culture) combined with high-resolution mass spectrometry. A total of 1,212 proteins were quantified in the exosomal proteome regardless of cell origin or isolation method. A group of 22 proteins was generally enriched. Compared to cells, 15 proteins were consistently decreased in the exosomal proteome. Among the enriched proteins, researchers identified proteins related to biogenesis, GTPases, and membrane proteins, such as CD47 and ITGB1. The missing protein groups in exosomes were primarily composed of nuclear proteins. The study identified syntenin-1 as a protein that is consistently abundant in exosomes from different cell origins. Syntenin-1 is also present in exosomes from various species and biological fluids, highlighting its potential use as a presumed universal exosome biomarker. This research provides a comprehensive quantitative map of core proteins universally present in exosomes, serving as a valuable resource for the scientific community.

 



Kugeratski, FG. Nat Cell Biol. 2021.

 

2. Proteomic Landscape of Exosomes Reveals the Functional Contributions of CD151 in Triple-Negative Breast Cancer

Triple-Negative Breast Cancer (TNBC) is an aggressive subtype of breast cancer. Due to limited molecular therapeutic targets, TNBC patients have poor overall survival rates. Recently, exosomes have been recognized as key mediators in cancer progression, but the molecular composition and functions of TNBC-derived exosomes remain unknown. The main objective of the study was to elucidate the proteomic landscape of serum exosomes from 10 TNBC patients and 17 healthy donors to identify potential therapeutic targets. Using a tandem mass tag-based quantitative exosome proteomics approach, researchers characterized the proteome of serum exosomes from individual patients, identified TNBC-specific exosomal protein features, and screened for differentially expressed proteins. Most importantly, researchers found that the expression levels of the tetraspanin protein CD151 were significantly higher in serum exosomes derived from TNBC patients compared to those from healthy subjects, and they validated these findings using samples from an additional 16 donors. Furthermore, they used quantitative proteomics to reveal the proteomes of CD151-deficient exosomes and cells, discovering that exosomal CD151 promotes the secretion of ribosomal proteins via exosomes while inhibiting the secretion of complement proteins through exosomes. Additionally, researchers demonstrated that CD151-deficient exosomes significantly reduced the migration and invasion of TNBC cells. This is the first comparative study of the proteomes of exosomes derived from TNBC patients and CD151-deficient exosomes. The results suggest that exosome proteomics of TNBC is a useful tool for understanding TNBC, and exosomal CD151 may be a potential therapeutic target for TNBC.

 



Li, S. et al. Mol Cell Proteomics. 2021.

 

Sample Submission Requirements



For more sample details, please consult our technical team.

 

Deliverables

1. Comprehensive Experimental Details

2. Materials, Instruments, and Methods

3. Relevant Liquid Chromatography and Mass Spectrometry Parameters

4. The Detailed Information of Exosome Proteomics

5. Mass Spectrometry Image

6. Raw Data