Glycohyocholic Acid Analysis Service

Glycohyocholic acid (GHCA) is a conjugated bile acid formed by the conjugation of hyocholic acid (HCA) with glycine. GHCA plays a crucial role in bile acid metabolism and gut microbiota interactions. Structurally, GHCA is characterized by hydroxy groups at the 3α, 6α, and 7α positions, which endow it with specific bioactive properties within bile acid metabolism. GHCA is synthesized in the liver through glycine conjugation and then secreted into bile, where it undergoes further metabolism by bacterial enzymes in the intestine. These distinct features make GHCA particularly valuable in studies of bile acid metabolism, liver health, and gut microbiota dynamics.

 

Figure 1. Molecular Structure of Glycohyocholic Acid

 

MtoZ Biolabs offers Glycohyocholic Acid Analysis Service using high performance liquid chromatography-mass spectrometry (HPLC-MS). This advanced method combines the separation capabilities of HPLC with the detection power of MS, ensuring high sensitivity and specificity in GHCA detection.

 

Analysis Workflow

 



 

Service Advantages

1. High Sensitivity

Detects very low concentrations of GHCA accurately.

 

2. High Specificity

Differentiates GHCA from other compounds using mass spectrometry.

 

3. Rapid Analysis

Efficient sample preparation and fast detection processes for high-throughput analysis.

 

4. High Accuracy

Strict quality control ensures accuracy and reproducibility.

 

5. Versatility

Capable of analyzing GHCA and its related metabolites.

 

Sample Submission Requirements

1. Sample Types

Serum, plasma, urine, feces, tissue extracts, and other biological samples.

 

2. Sample Volume

At least 200 μL of liquid sample or 200 mg of solid sample.

 

3. Sample Preservation

Store at -80°C to maintain stability.

 

Note: Provide details on sample collection and handling.

 

Deliverables

1. Experimental Procedures

2. Relevant Liquid Chromatography and Mass Spectrometry Parameters

3. Detailed Information on Glycohyocholic Acid

4. Raw Data

5. Custom Analysis Report

 

Our Glycohyocholic Acid Analysis Service provides high-quality data support, advancing your scientific research and applications. For any inquiries or requirements, please contact us.