Glycopeptide Mapping Service

Glycopeptide mapping is a mass spectrometry-based analytical method designed for precise structural characterization of glycosylated proteins. It aims to reveal the structural features of protein glycosylation, pinpoint glycosylation sites, and analyze the composition and heterogeneity of glycans. Glycopeptide mapping involves enzymatic digestion of protein samples into glycopeptides, followed by efficient enrichment techniques (such as affinity enrichment or metal oxide affinity enrichment) to capture target glycopeptides. Subsequently, liquid chromatography-mass spectrometry (LC-MS/MS) is used to separate and detect glycopeptides at high resolution, identifying glycosylation sites, glycan structures, and subtle glycosylation modifications. This analysis overcomes the limitations of traditional methods in detecting minor glycosylation differences, particularly in high-throughput studies of complex biological samples.

 



Figure 1. The Workflow of Glycopeptide Mapping

 

Glycopeptide mapping is not only useful for characterizing glycosylation patterns and ensuring quality control in biopharmaceuticals but also for identifying disease-related abnormal glycosylation patterns, supporting biomarker discovery and drug development research. Additionally, this method provides insights into the regulatory role of glycosylation in protein function, cellular signaling pathways, and disease progression, offering a deeper understanding of complex biological processes.

 

Services at MtoZ Biolabs

MtoZ Biolabs, an integrated chromatography and mass Spectrometry (MS) services provider, provides advanced proteomics, metabolomics, and biopharmaceutical analysis services to researchers in biochemistry, biotechnology, and biopharmaceutical fields. Our ultimate aim is to provide more rapid, high-throughput, and cost-effective analysis, with exceptional data quality and minimal sample consumption. MtoZ Biolabs offers a Glycopeptide Mapping Service that is designed to provide efficient and reliable glycopeptide analysis, ensuring seamless integration of the entire process from sample preparation and glycopeptide enrichment to mass spectrometry analysis and bioinformatics data interpretation, enabled by advanced mass spectrometry technology and a professional research team. Utilizing high-resolution mass spectrometry platforms and advanced algorithms, our glycopeptide mapping service deliver comprehensive insights into protein glycosylation distribution and characteristics, offering a one-stop solution for your sample analysis. If you are interested in our service, please feel free to contact us.

 

Service Advantages

1. Precise Characterization of Biopharmaceutical Glycosylation Profiles  

MtoZ Biolabs' glycopeptide mapping service employs advanced intact glycopeptide analysis techniques to thoroughly examine the glycosylation patterns of biopharmaceuticals. This ensures comprehensive and accurate assessment of glycosylation characteristics during production and quality control processes.  

 

2. One-Time-Charge

Our pricing is transparent, no hidden fees or additional costs.

 

3. High-Throughput Quantitative Analysis  

MtoZ Biolabs utilizes advanced glycan and glycopeptide quantitation techniques to achieve precise quantification of glycosylation sites and glycan structures in a high-throughput manner. Glycopeptide mapping service deliveres stable and reproducible quantitative data.

 

Case Study

1. A Highthroughput Workflow for Comprehensive Glycopeptide Mapping

This study presents a high-throughput workflow for comprehensive glycopeptide mapping, integrating efficient sample preparation, advanced mass spectrometry, and data analysis techniques. The approach enables detailed characterization of glycopeptides, including glycosylation sites and glycan heterogeneity, facilitating the study of glycoproteins in complex biological samples. Our glycopeptide mapping service utilizes cutting-edge mass spectrometry to provide high-throughput analysis of glycopeptides, offering accurate glycosylation site identification and glycan profiling to support your research needs.

 



Nat Biomed Eng. 2024.

Figure 2. Oxonium Ion Maps Generate a Specific and Quantitative Glycoproteome Analysis of Neat Human Plasma

 

2. Mapping the O-Glycoproteome Using Site-Specific  Extraction of O-linked Glycopeptides (EXoO)  

This study describes the EXoO (Extraction of O-linked glycopeptides) method, a site-specific approach for mapping the O-glycoproteome. The method combines selective enrichment of O-linked glycopeptides with advanced mass spectrometry to identify O-glycosylation sites and characterize glycan structures in complex biological samples. EXoO enables high-resolution analysis of O-glycoproteins, providing valuable insights into their structural and functional roles in biological systems. Our glycopeptide mapping service utilizes advanced enrichment techniques and mass spectrometry to identify O-glycosylation sites and glycan structures, offering precise and detailed insights into the glycoproteome for your research needs.

 



Yang, W. et al. Mol Syst Biol. 2018.

Figure 3. Mapping of O-linked Glycoproteome

 

Applications

1. Analysis of Glycosylation Sites and Glycan Structures  

Glycopeptide Mapping enables precise identification of glycosylation sites and glycan types in glycoproteins, providing critical structural information. Glycopeptide mapping service efficiently delivers detailed glycopeptide structural data to support related research.

 

2. Glycosylation Characterization in Drug Development  

In biopharmaceutical development, Glycopeptide Mapping is used to assess the glycosylation patterns of candidate drugs, ensuring consistency and efficacy. Glycopeptide mapping service provides reliable glycopeptide analysis support for drug development.

 

3. Disease Biomarker Research  

Glycopeptide mapping service can be used to detect disease-related abnormal glycosylation patterns, helping to identify potential glycoprotein biomarkers.

 

4. Glycosylation and Signal Transduction Research  

Glycopeptide mapping service explores complex biological processes through analyzing the regulatory role of glycosylation in protein function and signaling pathways.

 

Deliverables

1. Comprehensive Experimental Details

2. Materials, Instruments, and Methods

3. Relevant Liquid Chromatography and Mass Spectrometry Parameters

4. The Detailed Information of Glycopeptide Mapping

5. Mass Spectrometry Image

6. Raw Data