Glycoprotein Biomarker Discovery Service

Glycoprotein Biomarker Discovery Service utilizes advanced mass spectrometry and proteomics technologies to help researchers identify and analyze glycoproteins from complex biological samples, thereby discovering potential biomarkers. Glycoproteins play a crucial role in processes such as cell recognition, immune response, and signal transduction, and their glycosylation abnormalities are closely associated with the onset of various diseases, such as cancer, diabetes, and cardiovascular diseases. Therefore, discovering glycoprotein biomarkers is of great significance for early diagnosis, disease monitoring, and personalized treatment. Abnormal expression of glycoproteins often marks early changes in disease, providing reliable evidence for early diagnosis and helping to study the molecular mechanisms of diseases, offering new therapeutic targets. Furthermore, glycoprotein biomarkers can also be used to monitor treatment effectiveness, assess disease prognosis, and evaluate patient responses to treatment. By deeply studying the functions of glycoproteins and their roles in diseases, scientists can advance the development of precision medicine, improving early detection and treatment strategies. Thus, the discovery of glycoprotein biomarkers not only holds broad clinical application potential but also provides new insights for disease mechanism research and drug development.

 

MtoZ Biolabs, an integrated Chromatography and Mass Spectrometry Services Provider, provides advanced proteomics, metabolomics, and biopharmaceutical analysis services to researchers in biochemistry, biotechnology, and biopharmaceutical fields. We have established a powerful and professional glycoprotein biomarker discovery platform, which includes Thermo Fisher Q Exactive HF and Obitrap Fusion Lumos mass analyzer system, coupled with Nano-LC system. Our Glycoprotein Biomarker Discovery Service is applicable for early diagnosis, pathological mechanism research, personalized treatment planning, and new drug target discovery, driving the advancement of precision medicine and disease treatment strategies. Through high-sensitivity and precise analytical platforms, MtoZ Biolabs provides high-quality glycoprotein identification and quantitative analysis to meet the needs of both research and clinical applications.

 

Analysis Workflow

 



 

Service Advantages

1. Accurate Identification of Disease-Related Biomarkers: Our advanced mass spectrometry platform enables highly sensitive and specific identification of glycoprotein biomarkers associated with various diseases (e.g., cancer, cardiovascular diseases), assisting clients in the early detection of diseases.

 

2. Technological and Instrumentation Advantages: Leveraging world-class mass spectrometry instruments (Thermo Fisher Q Exactive HF and Orbitrap Fusion Lumos mass analyzers) and nano-scale liquid chromatography, our platform ensures precise detection of glycoprotein biomarkers, particularly suited for low-abundance samples.

 

3. Expert Team and Customized Services: Our highly experienced technical team provides fully customized solutions, from sample preparation to data analysis, tailored to meet the specific needs of clients in various research and application areas.

 

Sample Submission Suggestions

1. Sample Type: The service can analyze various sample types such as serum, plasma, tissue extracts, or cell culture supernatants. Samples should contain glycoproteins or glycosylated proteins.

2. Sample Purity: The sample should be as purified as possible to minimize contamination. Ideally, it should be enriched for glycoproteins if the target is specifically glycosylated biomarkers.

3. Sample Concentration: For optimal results, the protein concentration should generally be in the range of 0.5–5 mg/mL, depending on the analysis method and sample type.

4. Buffer Composition: Samples should be in a suitable buffer such as PBS, Tris, or other isotonic solutions. Avoid using high concentrations of salts or detergents that could interfere with glycoprotein analysis.

5. Storage Conditions: Samples should be stored at -80°C or -20°C for long-term storage. Avoid multiple freeze-thaw cycles to preserve the integrity of glycoproteins.

6. Special Instructions: If samples are from a specific biological source or treatment condition, please provide detailed information to optimize the biomarker discovery process.

 

If you need more detailed information or specific analytical methods, feel free to ask!

 

Sample Results

1. Serum Glycoprotein Biomarker Discovery and Qualification Pipeline Reveals Novel Diagnostic Biomarker Candidates for Esophageal Adenocarcinoma

 



Shah, AK. et al. Mol Cell Proteomics. 2015.

 

2. Discovery and Validation of Serum Glycoprotein Biomarkers for High Grade Serous Ovarian Cancer

 



Dutt, M. et al. Proteomics Clin Appl. 2023.

 

FAQ

1. How to prevent glycosylation loss during sample processing?
Non-denaturing buffers (e.g., PBS) and mild conditions are used during sample preparation to minimize glycan loss. Protectants like glycerol, protease inhibitors, and glycan-stabilizing agents are applied to prevent degradation of modifications. Simplifying processing steps and avoiding excessive washing or concentration helps reduce glycan damage risks.

 

2. How to address the challenge of detecting low-abundance glycoprotein biomarkers?
Enrichment of low-abundance glycoproteins can be achieved using lectin affinity chromatography, followed by nano-scale liquid chromatography and high-sensitivity mass spectrometry (e.g., Tribrid) to improve detection sensitivity. Techniques like TMT or SILAC labeling can amplify low-abundance signals, and software such as MaxQuant optimizes data processing by enhancing signal extraction and reducing noise.

 

Deliverables

1. Comprehensive Experimental Details

2. Materials, Instruments, and Methods

3. Relevant Liquid Chromatography and Mass Spectrometry Parameters

4. The Detailed Information of Glycoprotein Biomarker Discovery Analysis

5. Mass Spectrometry Image

6. Raw Data 

 

If you are interested in our Glycoprotein Biomarker Discovery Service, please feel free to contact us, we will serve you wholeheartedly.