Histamine Analysis Service

Histamine is an organic nitrogenous compound involved in local immune responses, as well as regulating physiological function in the gut and acting as a neurotransmitter for the brain, spinal cord, and uterus. It is primarily synthesized and stored in mast cells and basophils, and is released in response to allergens, leading to immediate hypersensitivity reactions. Therefore, precise and quantifiable analysis of histamine is crucial in various research areas, including allergy research, food industry, pharmacology, among others.

 

Additionally, it mediates inflammation, stimulates gastric acid secretion, and acts as a potent vasodilator. Because of its diverse physiological and pathological roles, alteration in histamine levels has been implicated in various diseases, such as allergic reactions, gastric ulcers, and pathological conditions, such as schizophrenia and depression. Therefore, histamine level detection and analysis are important.

 



 

Branco, A. C. C. C. et al. Mediators Inflamm. 2018.

 

Figure 1. Inflammatory and Regulatory Functions of Histamine on Different Body Sites

 

At MtoZ Biolabs, we offer comprehensive histamine analysis services using state-of-the-art mass spectrometry equipment. We have a professional team of well-trained scientists who can provide accurate and reliable measurement of histamine levels in various sample types. We ensure the highest accuracy and precision in our histamine analysis, thus giving you reliable data to draw conclusions about your research.

 

Analysis Workflow

1. Sample Preparation: Samples are prepared with meticulous attention to prevent histamine degradation.

2. Metabolite Extraction: Histamine is extracted using optimized extraction methods.

3. Mass Spectrometry Analysis: The extracted samples are analyzed using advanced high-performance liquid chromatography-mass spectrometry tandem (HPLC-MS/MS) techniques, ensuring accurate measurement of histamine levels.

4. Data Processing and Interpretation: Raw data are processed and analyzed by our expert bioinformaticians, providing you with reliable, interpretable results.

5. Report Generation: A comprehensive report with detailed analysis results and valuable insights is delivered to the clients.

 



 

Service Advantages

• Expertise: MtoZ Biolabs team consists of highly experienced scientists who have rich experience in mass spectrometry and metabolomics analysis.
• Advanced Techniques: We use high-resolution mass spectrometry for histamine analysis, which ensures high accuracy and precision.
• Customized Solutions: We provide customized solutions based on the specific requirements of individual projects.
• Comprehensive Service: We offer a complete solution from sample preparation to data analysis.

 

Applications

Our histamine analysis service can be applied in various fields, including but not limited to:

• Allergy research
• Food industry
• Pharmacological studies
• Clinical diagnosis

 

Sample Submission Requirements

1. Sample Types

We accept serum, plasma, urine, tissue, or other biological samples. A minimum of 3 samples under identical conditions is required.

 

2. Sample Volume

• Animal tissue: ≥ 100 mg
• Serum/ Plasma: ≥ 200 μL
• Urine: ≥ 2 mL

 

3. Sample Preservation

Samples must be stored at -80°C to ensure stability.

 

Note: Please provide precise details about sample collection and handling.

 

Deliverables

1. Experimental Procedures

2. Relevant Liquid Chromatography and Mass Spectrometry Parameters

3. Detailed Information on Histamine

4. Raw Data

5. Custom Analysis Report