How Many Proteins Can Be Detected by TMT in Proteomics
Tandem Mass Tag (TMT) is a widely used high-throughput protein quantification technique in proteomics, designed to investigate protein expression differences across various samples. TMT enables the quantitative comparison of corresponding proteins between samples by labeling peptides with specific tags following protein hydrolysis.
Detection Capacity of TMT Technology
TMT technology is capable of accurately and efficiently determining the relative abundance of thousands of proteins. Typically, depending on the specific experimental design and instrumentation, TMT can detect approximately 8000 to 10000 proteins. This capability facilitates a comprehensive analysis of protein expression variations between different samples, such as those between normal and diseased tissues.
Biomedical Applications of TMT Technology
TMT technology is extensively utilized in the field of biomedical research. For example, comparing protein expression profiles between diseased and normal tissues can lead to the identification of potential disease biomarkers or proteins associated with specific diseases. Moreover, TMT enables the study of post-translational modifications, thereby elucidating protein functionality and regulatory mechanisms.
With its broad applicability in proteomics, TMT technology serves as a vital tool for in-depth understanding of protein composition and functionality in biological systems. However, it is important to consider that specific detection capabilities may vary based on experimental conditions, sample quality, and other factors.
MtoZ Biolabs, an integrated chromatography and mass spectrometry (MS) services provider.
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