How Much Protein Is Needed for Edman Sequencing?

In Edman protein sequencing, the amount of protein typically needed depends on various factors, such as the sensitivity of the sequencing equipment, the purity of the protein, and the complexity of the sequence. Traditionally, the Edman degradation method is used to determine the amino acid sequence of proteins or polypeptides, usually requiring about 1 to 100 picomoles (pmol) of protein sample. This is equivalent to approximately 0.1 to 10 micrograms (μg) of protein, depending on the molecular weight of the protein.

 

For regular Edman sequencing, about 10-100 picomoles (pmol) of protein sample are usually required. This is equivalent to a few hundred nanograms to a few micrograms of protein. However, with modern, high-sensitivity equipment, this amount may be even less, potentially reaching below the single picomole level.