How to Interpret Glycan Analysis?

Glycan Analysis is a method used to determine the structure of glycan chains on proteins or other large biomolecules. This analysis can be quite complex due to the high diversity and complexity of glycan structures. Here are some key aspects that are generally focused on during glycan analysis.

 

Glycan Composition

Determining which monosaccharide units are present in the glycan, such as glucose, galactose, N-acetylglucosamine, and others.

 

Glycan Length

Analyzing the length of the sugar chain in carbohydrate compounds, i.e., the number of sugar units.

 

Glycan Linkage

The monosaccharide units in the glycan are connected by glycosidic bonds. The types (α or β) and positions (e.g., 1-4 or 1-6 linkages) of these bonds determine the structure and function of the glycan.

 

Spatial Configuration

Analyzing the spatial arrangement of monosaccharide units, such as D-form or L-form configurations.

 

Branching Structure

Glycans can be straight chains or branched chains. The presence and type of branching are crucial for the biological function of the glycan.

 

Post-Translational Modifications

Glycans may undergo various modifications, such as phosphorylation, sulfation, and acetylation.