HPLC Tandem Mass Spectrometry Service

HPLC tandem mass spectrometry is an advanced analytical technique that combines the high-resolution separation capability of liquid chromatography with the high-sensitivity detection of mass spectrometry, enabling the precise separation of individual components within complex samples and offering detailed qualitative and quantitative data. This makes it an ideal tool for processing complex protein samples in diverse fields such as proteomics, clinical research, drug development, and biomarker screening.

 

MtoZ Biolabs offers HPLC tandem mass spectrometry service supported by state-of-the-art mass spectrometry platforms, including Thermo Fisher’s Q Exactive HF, Orbitrap Fusion, Orbitrap Fusion Lumos, and others, as well as advanced liquid chromatography systems like Nano-LC. These platforms ensure efficient separation and precise analysis of your samples. Whether you're conducting proteomics research, protein quantification studies, or investigating low-abundance protein variations, we deliver reliable and accurate data to support your scientific and development goals.

 

Technical Principles

HPLC tandem mass spectrometry combines the high separation power of liquid chromatography with the sensitivity of mass spectrometry, enabling the analysis of complex biological samples. In this process, samples are first separated by HPLC, where components are isolated based on their chemical properties (e.g., polarity, molecular weight). The separated components are then identified and analyzed by mass spectrometry, where their mass-to-charge ratios are measured, providing both qualitative and quantitative data.

 

In tandem mass spectrometry mode, a parent ion is first isolated in the mass spectrometer. It then undergoes fragmentation in the collision cell, producing smaller fragment ions. By analyzing the mass-to-charge ratios (m/z) of these fragments, detailed structural information about the molecule can be obtained. Combined with HPLC’s high separation capacity, this enables precise and reliable analysis of complex samples. This technology not only allows for accurate quantification of low-abundance substances, but also works across a broad range of sample types, including proteins, metabolites, and small molecules. HPLC tandem mass spectrometry is a cornerstone technology in proteomics, metabolomics, and other areas, with extensive potential applications.

 

 

Hristova, J. et al. Biotechnol. Biotechnol. Equip. 2022.

 

Services at MtoZ Biolabs

MtoZ Biolabs offers a comprehensive range of HPLC tandem mass spectrometry service to help researchers and companies gain in-depth insights into protein structure, function, and dynamic changes. Our platform covers the following core services:

1. Protein Qualitative Analysis

Utilizing HPLC tandem mass spectrometry, we accurately identify proteins and their variants, including molecular weight, amino acid sequences, and modification states. This service is essential in basic research, drug target validation, and clinical biomarker discovery.

 

2. Protein Quantitative Analysis

We offer highly sensitive quantitative analysis to precisely measure changes in protein abundance—either relative or absolute—under various experimental conditions. This service provides critical data for protein expression profiling, disease studies, and drug evaluation.

 

3. Proteomics Analysis

We conduct large-scale proteomics studies that provide comprehensive data on protein types, abundance, and functions. By integrating high-throughput data analysis, we enable clients to better understand protein composition and its biological significance in cells, tissues, or body fluids.

 

4. Post-Translational Modification (PTM) Analysis

Our HPLC tandem mass spectrometry service extends to the analysis of protein post-translational modifications (such as phosphorylation, acetylation, and glycosylation), providing insights into protein function regulation. This service is crucial for disease mechanism research, signal pathway studies, and drug development.

 

5. Protein Structural Analysis

Through a combination of mass spectrometry and liquid chromatography, we can resolve the three-dimensional structure of proteins, shedding light on their spatial conformation and functional relevance. This analysis supports drug discovery, protein function research, and the development of targeted therapeutic strategies.

 

Why Choose MtoZ Biolabs?

1. Advanced Analysis Platform: MtoZ Biolabs established an advanced HPLC tandem mass spectrometry platform, guaranteeing reliable, fast, and highly accurate analysis service.

2. One-Time-Charge: Our pricing is transparent, no hidden fees or additional costs.

3. High-Data-Quality: Deep data coverage with strict data quality control. AI-powered bioinformatics platform integrate all protein analysis data providing clients with a comprehensive data report.

4. Multi-Sample Compatibility: Our platform supports a wide variety of biological samples, including cells, tissues, blood, and bodily fluids, providing flexibility to accommodate diverse research demands.

5. Customized Service: Tailored to the specific research needs of our clients, we offer flexible experimental design and personalized data analysis to ensure the achievement of research goals to the fullest extent.

 

Sample Submission Suggestions

To ensure successful execution of the HPLC tandem mass spectrometry service and obtain accurate results, sample quality and preparation are critical. We recommend that clients provide samples meeting the following criteria to ensure precise analysis and reliable data.

• Low-complexity samples: Gel spots or bands, approximately 1-2 cm in length.
• Medium-complexity samples: Protein mixtures, including column-purified samples.
• Moderate-to-high complexity samples: Protein mixtures such as IP-derived samples, secreted proteomes, or subcellular organelles (e.g., mitochondria, endoplasmic reticulum, lysosomes).
• High-complexity samples: Whole cell lysates, tissues.

 

For more specific sample submission requirements, please consult our technical team.

 

Case Study

Case 1

HPLC tandem mass spectrometry successfully analyzed digestion products from different protein sources, revealing their digestion and intestinal absorption potential.

 

 Sousa, R. et al. Food Res. Int. 2020.

 

Case2

HPLC tandem mass spectrometry revealed TRIM59's role in pulmonary hypertension through regulation of protein ubiquitination.




Liu, Y. L. et al. J. Transl. Med. 2023.

 

 

Deliverables

1. Comprehensive Experimental Details

2. Materials, Instruments, and Methods

3. Total Ion Chromatogram & Quality Control Assessment

4. Data Analysis, Preprocessing, and Estimation

5. Bioinformatics Analysis

6. Raw Data Files

 

MtoZ Biolabs offers efficient and accurate HPLC tandem mass spectrometry service, committed to providing comprehensive data support for your research. We also offer a wide range of proteomics, metabolomics, and small molecule analysis services. Contact us for more information and professional advice.