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    Indole-3-acetic Acid Analysis Service

      Indole-3-acetic acid (IAA) is a prominent plant hormone belonging to the auxin family, playing a crucial role in regulating plant growth and development. Structurally, IAA is a simple indole derivative (C₁₀H₉NO₂) characterized by an indole ring fused to a carboxymethyl group at position 3. Its molecular structure is instrumental in its ability to influence cellular processes such as cell elongation, division, and differentiation. IAA is primarily synthesized in the apical meristems of plants, with tryptophan serving as the principal precursor. It is widely distributed in plants, with varying concentrations observed in different tissues, reflecting its multifaceted roles in plant physiology.

       

      IAA's influence extends across a variety of plant processes, including embryogenesis, root and shoot development, and responses to environmental stimuli. Its ability to regulate gene expression and modulate cell wall properties makes it indispensable for maintaining plant architecture and adapting to changing environmental conditions. Research has demonstrated that IAA operates through complex signaling pathways, involving the perception by TIR1/AFB receptors and subsequent transcriptional changes mediated by AUX/IAA proteins. These pathways underscore the importance of IAA in the intricate hormonal network governing plant life.

       

      Given its pivotal role in plant biology, the study of IAA is critical for advancing our understanding of plant growth mechanisms and improving agricultural practices. Quantitative and qualitative analysis of IAA levels in plants can provide insights into developmental stages, stress responses, and the efficacy of agricultural treatments. Furthermore, IAA's interactions with other phytohormones such as cytokinins, gibberellins, and abscisic acid highlight its central role in the hormonal crosstalk that fine-tunes plant responses to both internal and external cues.

       

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      Figure 1. The Structure of Indole-3-acetic Acid

       

      At MtoZ Biolabs, we provide comprehensive Indole-3-acetic Acid Analysis Service using our state-of-the-art mass spectrometry platforms. Our experienced scientific team can aid you in uncovering the intricate role of IAA in your research. Our expert team at MtoZ Biolabs can help you:

      • Accurately quantify the level of IAA in various sample types.
      • Determine the distribution and metabolism of IAA.
      • Identify potential correlations between IAA levels and plant phenotypes or diseases.

       

      Analysis Workflow

       

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      Service Advantages

      • Highly Sensitive and Accurate: Our LC-MS/MS platform can detect IAA at low concentrations with high accuracy.
      • Wide Range of Sample Types: We accept a variety of sample types for IAA analysis.
      • Professional Team: Our team of experienced scientists ensures reliable results.
      • Comprehensive Report: We provide a detailed analysis report with both raw and processed data, methodology, and results interpretation.

       

      Applications

      Our IAA analysis service can be applied in various research areas, including:

      • Plant biology and agriculture
      • Environmental science
      • Plant-microbe interactions
      • Plant pathology and disease control research

       

      Sample Submission Requirements

      • Sample type: Fresh plant tissues, seeds
      • Sample amount: ≥ 1 g
      • Storage and Shipping: Samples should be stored at -80℃ and shipped with dry ice.
      • Avoid repeated cycles of freezing and thawing
      • More than 3 biological replicates should be prepared.

      For more details, please feel free to get in touch with our customer service team.

       

      Deliverables

      1. Experimental Procedures

      2. Relevant Liquid Chromatography and Mass Spectrometry Parameters

      3. Detailed Information on Indole-3-acetic Acid

      4. Raw Data

      5. Custom Analysis Report

       

      At MtoZ Biolabs, we are committed to providing reliable, high-quality services to support your research. For any inquiries or further information regarding our Indole-3-acetic Acid Analysis Service, please do not hesitate to contact us.

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