Indole-3-lactic Acid Analysis Service
- State-of-the-art HPLC-MS/MS systems
- Highly experienced analytical team
- Rigorous data analysis and quality control
- Comprehensive report with both raw data, processed data and interpretation of the results
- Competitive pricing and fast turnaround time
- Samples should be flash-frozen in liquid nitrogen immediately after collection and stored at -80°C.
- Quantity: At least 200 μl liquid, 1 x 10⁷ cells, or 200 mg tissue.
- The sample should be shipped on dry ice.
- More than 3 biological replicates should be prepared.
Indole-3-lactic acid (ILA) is a significant metabolite within the tryptophan metabolism pathway, characterized by its indole ring structure conjugated to a lactic acid moiety. This compound is produced by various microorganisms, including lactic acid bacteria such as Lactobacillus and Bifidobacterium species, as well as by plants and animals. ILA has garnered considerable interest due to its diverse biological activities, including its roles in modulating immune responses, maintaining gut health, and exerting antimicrobial effects. In the human gut, ILA contributes to the balance of the microbiome and interacts with the host immune system, influencing conditions such as inflammatory bowel disease (IBD), colorectal cancer (CRC), and even neurological disorders like autism spectrum disorder (ASD).
Recent research has highlighted the multifaceted roles of ILA across different biological systems. In plants, ILA is involved in growth regulation and stress responses. In humans, its impact on the gut-brain axis and immune modulation has been increasingly recognized, with studies showing that ILA can inhibit pathogenic bacterial growth and reduce inflammation in the gastrointestinal tract. These findings suggest that ILA plays a crucial role in maintaining homeostasis within complex biological environments, making it a valuable target for both basic and applied research.
The importance of ILA research extends to various fields, including microbiology, immunology, and metabolomics. Understanding the pathways and mechanisms through which ILA exerts its effects can lead to the development of new therapeutic strategies for managing gut-related diseases, enhancing crop resilience in agriculture, and exploring novel probiotic treatments for improving human health. Given its broad implications, precise and sensitive analysis of ILA is essential for advancing research and clinical applications.
Figure 1. Schematic Representation of How The Probiotic Strain Lactobacillus Plantarum L168 and Its Metabolite, Indole-3-lactic Acid, Ameliorate Intestinal Inflammation, Tumor Growth, and Gut Dysbiosis
MtoZ Biolabs provides a comprehensive Indole-3-lactic Acid Analysis Service, utilizing advanced HPLC-MS/MS platforms for the sensitive and reliable quantification of ILA in different sample types. Our service is designed to meet the needs of both academic and pharmaceutical industry researchers, providing you with accurate, reliable, and reproducible results.
Analysis Workflow
Our analysis procedure includes the following steps:
1. Sample Preparation: We accept a variety of sample types, including but not limited to plant tissues, plasma, serum, cell, etc.
2. Metabolite Extraction: We use optimized protocols to extract metabolites from your samples.
3. HPLC-MS/MS Analysis: We employ high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) for precise metabolite identification and quantification.
4. Data Analysis: We provide comprehensive data analysis, including peak identification, quantification, and statistical analysis.
5. Report: We deliver a detailed report that includes a summary of the experimental procedure, data analysis, and results.
Service Advantages
Sample Submission Requirements
We can analyze a variety of biological materials, including but not limited to plant tissues, plasma, serum, cell, etc. To ensure the validity and reliability of the results, we have set the following sample submission requirements:
For detailed information on sample submission, please contact our customer service team.
Deliverables
1. Experimental Procedures
2. Relevant Liquid Chromatography and Mass Spectrometry Parameters
3. Detailed Information on Indole-3-lactic Acid
4. Raw Data
5. Custom Analysis Report
For more details on our Indole-3-lactic Acid Analysis Service, please do not hesitate to contact us.
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