IP-Mass Spectrometry Service

MtoZ Biolabs is dedicated to providing high-quality proteomics analysis services for life sciences research, with IP-Mass Spectrometry technology being one of our core services. IP-Mass Spectrometry is a powerful tool for studying protein interaction networks, enabling precise identification of the components in immunoprecipitated complexes and their post-translational modification (PTM) states. This technology finds wide applications in signaling pathway analysis, protein complex function research, and disease mechanism exploration. Leveraging the Thermo Fisher Orbitrap Fusion Lumos and Nano-LC systems, MtoZ Biolabs offers high-sensitivity, high-resolution IP-Mass Spectrometry service to help your research projects achieve innovative breakthroughs.

 

Technical Principles

IP-Mass Spectrometry combines the specificity of immunoprecipitation (IP) with the high sensitivity of mass spectrometry, making it a powerful tool for analyzing protein interactions and PTMs. In this process, specific antibodies bind to target proteins and their associated partners through protein A/G magnetic beads or resin, selectively enriching the complexes while maintaining their native states. The captured protein complexes are then eluted and enzymatically digested into peptides, which are analyzed by high-resolution mass spectrometry to determine their mass and sequence. Database analysis allows IP-Mass Spectrometry to precisely identify proteins, analyze their interactions, and reveal their PTM states, providing crucial insights into complex biological processes.

 



Brinkmalm, G. et al. J Mass Spectrom. 2012.

 

Technical Advantages

MtoZ Biolabs' IP-Mass Spectrometry service stands out by combining cutting-edge mass spectrometry capabilities with precise immunoprecipitation techniques, enabling comprehensive analysis of protein interactions and modifications with unmatched accuracy.

• High Sensitivity: Enables accurate detection of low-abundance interacting proteins.
• High Specificity: Strong antibody-binding specificity ensures the purity of target protein complexes.
• Rich Data: Provides interaction protein information as well as PTM states.
• Broad Applicability: Suitable for various biological samples, including cells, tissues, and bodily fluids.

 

Analysis Workflow

The IP-Mass Spectrometry service features a meticulously crafted workflow that ensures the integrity of the experimental process while maximizing data accuracy. From sample preparation to advanced bioinformatics, each step is designed to uncover key insights into protein interactions.

1. Sample Preparation: Standardized protocols ensure protein stability and integrity, ensuring robust immunoprecipitation outcomes.
2. Target Enrichment: Specific antibodies immobilized on magnetic beads capture target proteins and complexes with minimal background interference.
3. Mass Spectrometry: Digested peptides undergo high-resolution analysis using advanced Nano-LC and Orbitrap systems.
4. Data Reporting: Bioinformatics analysis delivers detailed protein identifications, interaction networks, and PTM insights in a comprehensive report.

 

Why Choose MtoZ Biolabs?

1. Advanced Analysis Platform: MtoZ Biolabs established an advanced IP-Mass Spectrometry platform, guaranteeing reliable, fast, and highly accurate analysis service.

2. One-Time-Charge: Our pricing is transparent, no hidden fees or additional costs.

3. High-Data-Quality: Deep data coverage with strict data quality control. AI-powered bioinformatics platform integrate all proteomics data, providing clients with a comprehensive data report.

4. Customized Service: Tailored to the specific research needs of our clients, we offer flexible experimental design and personalized data analysis to ensure the achievement of research goals to the fullest extent.

 

Applications

The IP-Mass Spectrometry service at MtoZ Biolabs is an essential tool for exploring protein interactions and post-translational modifications. Its broad utility makes it an ideal solution for a wide range of research applications that demand precision and depth.

• Signaling Pathway Studies: Uncover protein interactions critical to signal transduction.
• Protein Complex Analysis: Determine natural protein complex composition and functions.
• Disease Mechanism Research: Map interaction networks related to disease and identify potential therapeutic targets.
• PTM Exploration: Investigate how PTMs regulate protein activity and interactions.

 

Case Study

Case1

IP-Mass Spectrometry was used to analyze the phosphorylation dynamics of MPK4 and its interacting proteins in plant immunity, revealing its critical role in immune signal transduction.

 



Zhang, T. et al. J. Proteome Res. 2019.

 

 

Case2

IP-Mass Spectrometry analyzed VP40-derived peptide variants to precisely quantify the species-specific loads of circulating Ebola virus, aiding in virus diagnosis and load monitoring.

 



Shu, Q. B. et al. PLOS Pathog. 2021.

 

 

FAQ

Q1: How can non-specific binding in immunoprecipitation be minimized for mass spectrometry analysis?

Non-specific binding is a common issue in immunoprecipitation that may lead to background protein interference. MtoZ Biolabs minimizes non-specific binding by optimizing experimental conditions, including selecting high-quality specific antibodies, strictly controlling the antibody-to-bead ratio, and implementing thorough washing steps. Additionally, stringent background filtering and control group analysis of mass spectrometry data ensure specificity and reliability of the results.

 

Q2: Is IP-Mass Spectrometry effective for proteins expressed at low levels?


Enriching and detecting low-abundance target proteins is a significant technical challenge. Our IP-Mass Spectrometry service optimizes enrichment strategies, such as using high-sensitivity antibodies and improved immunoprecipitation protocols, to maximize the capture efficiency of target proteins. Combined with the ultra-high sensitivity of the Thermo Fisher Orbitrap Fusion Lumos mass spectrometer, we deliver reliable identification of low-abundance proteins and provide detailed PTM analyses.

 

Deliverables

1. Comprehensive Experimental Details

2. Materials, Instruments, and Methods

3. Total Ion Chromatogram & Quality Control Assessment

4. Data Analysis, Preprocessing, and Estimation

5. Bioinformatics Analysis

6. Raw Data Files

 

With state-of-the-art technology and a seasoned team, MtoZ Biolabs provides tailored IP-Mass Spectrometry solutions to advance your protein interaction research. Reach out today for customized support.