Isobaric Labeling Mass Spectrometry
Isobaric labeling mass spectrometry is a technique that introduces isotopic labels into specific biomolecules via chemical methods, facilitating quantitative and qualitative analysis using mass spectrometry. This approach finds broad applications in proteomics, particularly in the study of differential proteomics and dynamic protein changes. As biomedical research advances, the need for precise quantification and identification of proteins in complex biological systems is growing. By introducing known isotopic labels, isobaric labeling mass spectrometry allows for the distinction between original and labeled molecules during detection, enabling high-precision quantitative analysis. This method addresses the challenges of sample complexity and sensitivity typical in traditional mass spectrometry, especially when comparing protein expression under varying biological conditions. It supports both relative and absolute quantification, offering an effective tool for exploring protein functions and dynamics within biological systems. In disease research, such as cancer, this technique allows for the identification and quantification of biomarkers, aiding in early diagnosis, classification, and treatment monitoring. In drug development, it assists in drug target identification, investigation of metabolic pathways, and elucidation of drug mechanisms.
There are three primary methods for implementing isobaric labeling mass spectrometry: stable isotope labeling with amino acids in cell culture (SILAC), isobaric labeling (ICAT, TMT, iTRAQ, etc.), and isotopic labeling through chemical derivatization (e.g., DIGE). SILAC involves incorporating stable isotope-labeled amino acids during cell culture to achieve comprehensive protein labeling, representing a classic approach for in vivo isotopic labeling. Isobaric labeling connects isotopic tags directly to proteins or peptides via chemical reactions, eliminating the need for cell culture and being suitable for various sample types. Chemical derivatization involves labeling proteins or peptides chemically, facilitating the differentiation of sample sources and quantities in mass spectrometry analysis.
MtoZ Biolabs offers mass spectrometry analysis services aimed at providing clients with high-precision and high-sensitivity protein analysis solutions. Our technical team, with extensive experience in proteomics research, can design optimal experimental plans and deliver detailed analysis reports tailored to clients' research needs. Partnering with MtoZ Biolabs ensures access to advanced technical support and professional customer service.
MtoZ Biolabs, an integrated chromatography and mass spectrometry (MS) services provider.
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