iTRAQ-based Quantitative Proteomics Analysis
- Sample Types: Blood, urine, tissues, fungi, cerebrospinal fluid and other samples.
- Quantity: At least 200 μl liquid, 1 x 10⁷ cells, or 200 mg tissue.
iTRAQ, which stands for Isobaric Tags for Relative and Absolute Quantitation, has revolutionized quantitative proteomics with its ability to simultaneously identify and quantify proteins across multiple samples with exceptional precision and versatility. At MtoZ Biolabs, we offer comprehensive iTRAQ-based Quantitative Proteomics Analysis Services to support your research needs in advanced proteomic profiling and biomarker discovery.
iTRAQ-based quantitative proteomics is a technique that employs isobaric tagging reagents to label peptides from different protein samples. The iTRAQ reagents are chemically identical in mass but release reporter ions of distinct masses during tandem mass spectrometry (MS/MS). This allows for the simultaneous identification and quantification of proteins from multiple samples within a single liquid chromatography-mass spectrometry (LC-MS/MS) run, making it a powerful tool for comparative proteomic studies.
iTRAQ consists of a peptide-reactive group, a balance group, and a reporter group. The reactive group covalently binds to peptides, ensuring consistent and uniform labeling. The balance group offsets the mass added by the reporter group, so all labeled peptides have identical overall masses. The reporter group enables protein quantification by indicating the relative abundance of each peptide from different samples. In the initial MS1 scan, these peptides appear as single peaks due to their equal masses. During MS2 analysis, fragmentation causes the balance group to be lost neutrally, while the reporter group is released as a reporter ion with a unique mass specific to each sample's tag. By measuring the intensities of these reporter ions, the relative abundance of peptides across samples can be accurately determined. This allows precise quantification of proteins, making iTRAQ-based proteomics a powerful tool for comparative protein analysis.
Figure 1. Structure of iTRAQ Tag
Figure 2. The Process of iTRAQ Peptide Labeling
Services at MtoZ Biolabs
iTRAQ-based proteomics has emerged as a fundamental technology in the life sciences, offering unparalleled precision, consistency, and comprehensive protein profiling. MtoZ Biolabs is devoted to providing high-quality iTRAQ-based quantitative proteomics analysis services. We tailor our solutions to meet the specific needs of researchers and scientists, ensuring reliable and insightful data that drive innovation and support groundbreaking discoveries.
Analysis Workflow
Abudouwayiti, A. et al. Sci Rep. 2021.
Figure 3. Workflow of iTRAQ-based Quantitative Proteomics Analysis
Why Choose MtoZ Biolabs?
1. Advance Analysis Platform: MtoZ Biolabs established an advanced iTRAQ-based quantitative proteomics analysis platform, guaranteeing reliable, fast, and highly accurate analysis service.
2. One-Time-Charge: Our pricing is transparent, no hidden fees or additional costs.
3. High-Data-Quality: Deep data coverage with strict data quality control. AI-powered bioinformatics platform integrate all iTRAQ-based quantitative proteomics data providing clients with a comprehensive data report.
4. High Multiplexing Capability: iTRAQ technology allows for the simultaneous quantification of proteins across up to 10 different samples in a single experiment, increasing throughput and efficiency.
5. Consistency and Reproducibility: Labeling peptides prior to sample mixing minimizes variability introduced during sample preparation and analysis, ensuring consistent results across experiments.
Applications
1. Biomedical Research: Biomarker Discovery, Drug Development, Clinical Diagnostics
2. Basic Biological Research: Post-translational Modifications, Comparative Proteomics, Systems Biology
3. Agriculture and Food Science
4. Industrial and Environmental Applications
Sample Submission Suggestions
*Note: For other sample types, please contact us for more details.
Deliverables
1. Comprehensive Experimental Details
2. Materials, Instruments, and Methods
3. Total Ion Chromatogram & Quality Control Assessment (project-dependent)
4. Data Analysis, Preprocessing, and Estimation (project-dependent)
5. Bioinformatics Analysis
6. Raw Data Files
MtoZ Biolabs, an integrated Chromatography and Mass Spectrometry (MS) Services Provider, provides advanced proteomics, metabolomics, and biopharmaceutical analysis services to researchers in biochemistry, biotechnology, and biopharmaceutical fields. Our ultimate aim is to provide more rapid, high-throughput, and cost-effective analysis, with exceptional data quality and minimal sample consumption. If you are interested in our iTRAQ-based quantitative proteomics analysis service, please feel free to contact us.
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