L-Isoleucine Analysis Service

L-Isoleucine is an essential amino acid and one of the branched-chain amino acids (BCAAs), playing multiple crucial physiological roles in the body. It is involved in protein synthesis, energy metabolism, and muscle repair through various metabolic pathways. L-Isoleucine helps regulate blood sugar levels, promotes growth, and repairs tissues. It can be converted into acetyl-CoA and succinyl-CoA, which are involved in energy production and anabolic processes. Analyzing L-isoleucine metabolism can provide a better understanding of its mechanisms of action in various physiological and pathological states. Given its critical roles in immune and nervous system health, precise analysis of L-isoleucine and its metabolites is essential for understanding their functions and metabolic pathways in the body.

 



Figure 1. Molecular Structure of L-Isoleucine

 

MtoZ Biolabs utilizes liquid chromatography-tandem mass spectrometry (LC-MS/MS) to conduct detailed quantitative and qualitative analyses of L-isoleucine and its metabolites. This advanced method integrates LC's rapid separation capabilities with the high detection sensitivity of MS/MS, facilitating the precise measurement of L-isoleucine in complex biological matrices.

 

Analysis Workflow

 

 

 

Service Advantages

1. High Sensitivity and Precision

HPLC-MS/MS provides highly sensitive and accurate measurements, suitable for detecting low concentrations of L-isoleucine and differentiating its isomers.

 

2. Rapid and Efficient

Optimized sample preparation and fast analysis ensure quick and efficient processing.

 

3. Wide Applicability

Suitable for various biological samples, including blood, urine, and tissues.

 

4. Accurate and Reliable Data

Ensures high reproducibility and low error rates, supporting both research and clinical applications, providing reliable and specific results.

 

Sample Submission Requirements

Please ensure that the sample volume is greater than 0.2 g or 0.2 mL.

 

Applications

Clinical Research: Monitor L-isoleucine levels to study its roles in metabolic diseases, immune function, and neurological disorders.

 

Disease Monitoring: Analyze L-isoleucine and its metabolites to monitor disease progression, such as diabetes, muscle atrophy, and liver disease.

 

Nutritional Research: Evaluate the L-isoleucine content in foods and supplements to guide nutritional intake and health management.

 

Drug Development: Investigate the potential applications of L-isoleucine and its metabolites in new drug development, assessing efficacy and safety.

 

Deliverables

1. Experimental Procedures

2. Relevant Liquid Chromatography and Mass Spectrometry Parameters

3. Detailed Information on L-Isoleucine and their Metabolite

4. Raw Data

5. Custom Analysis Report

 

MtoZ Biolabs is dedicated to advancing your research and clinical projects by providing detailed insights into L-isoleucine's extensive physiological roles through top-tier analytical techniques and expert services.