Label-Free Quantitative Proteomic Analysis of Escherichia Coli

Escherichia coli (E. coli), as a member of the gut microbiome, plays an extremely important role in environmental monitoring, disease models, genetic research, and the production of biological products. In recent years, with the development of proteomics technology, the Label Free quantitative proteomics analysis method has become a powerful tool for studying cellular protein expression and function. This article aims to elaborate on the E. coli Label Free quantitative proteomics analysis service provided by MtoZ Biolabs.

 

Analysis Workflow

1. Sample Preparation

The process begins with protein extraction, ensuring protein stability during sample processing to prevent degradation. Then the protein is digested, usually with trypsin, to break the protein down into peptides, preparing for mass spectrometry analysis.

 

2. Mass Spectrometry Analysis

The peptides are introduced into a mass spectrometer for ionization and then detected and analyzed based on their mass-to-charge ratio (m/z). The Label Free method particularly relies on high-resolution and high-precision mass detection.

 

3. Data Processing and Quantitative Analysis

The mass spectrometry data are processed by professional bioinformatics software that can recognize peptides and map them back to their source proteins. Quantitative analysis is based on the signal intensity of the peptides, without the need for any chemical labeling, directly reflecting the abundance of the proteins.

 

Application of Label Free Technology in E. coli Research

Through Label Free technology, researchers can directly observe the changes in protein expression in E. coli under different growth conditions, different genotypes, or under the effect of drugs. For example, comparing the proteome of E. coli at different temperatures can reveal the temperature response mechanism. In addition, the analysis of the differences in protein expression between antibiotic-resistant strains and sensitive strains can help identify new drug targets or resistance mechanisms.

 

Comparison of Label Free and Other Proteomics Analysis Techniques

Compared with traditional labeling methods (such as SILAC and iTRAQ), Label Free technology simplifies the experimental process, reduces costs, and minimizes potential bias caused by labeling. Nevertheless, each method has its suitable scenarios. Label Free analysis is suitable for samples with large quantities and small variability, while labeling methods are suitable for samples with high complexity or those requiring high sensitivity detection.

 

MtoZ Biolabs' Label Free proteomics analysis service provides not only high-throughput and high-precision analysis capabilities but also advanced data processing software and a professional technical support team. Collaborating with MtoZ Biolabs, we will help you quickly and accurately unlock the secrets of Label Free technology. Feel free to contact us to learn more details!