LC-MS Protein Quantification Service
- Cell or tissue lysates
- Serum and plasma
- Cell culture media
- Tissue homogenates
- Other biological samples (e.g., urine, saliva)
The LC-MS Protein Quantification Service utilizes liquid chromatography-mass spectrometry (LC-MS) to perform quantitative analysis of protein samples. This service employs LC to efficiently separate complex samples and MS to accurately detect the abundance of target proteins or peptides. LC-MS Protein Quantification Service delivers high sensitivity, specificity, and throughput, making it ideal for studying changes in protein expression levels within biological systems.
Protein quantification is a critical component of proteomics research, particularly in drug development, disease studies, and fundamental biological research. Accurate protein concentration data provides a reliable foundation for subsequent functional analysis, interaction studies, and biomarker discovery. Traditional protein quantification methods, such as the BCA assay, Bradford assay, and SDS-PAGE, are widely used in routine laboratory work. However, these methods are often limited by sample complexity, sensitivity, and accuracy. Unlike traditional techniques, LC-MS-based protein quantification offers superior sensitivity, selectivity, and precision, particularly when analyzing complex samples. This makes it an unparalleled tool for generating robust and detailed quantitative data in proteomics research.
van den Broek I. et al. Bioanalysis. 2015.
Figure1. Strategies for the Quantification of Intact Proteins by LC-MS
Service at MtoZ Biolabs
Leveraging an advanced LC-MS/MS technology platform, including the Thermo Fisher Q Exactive HF and Orbitrap Fusion Lumos mass spectrometer systems combined with Nano-LC, MtoZ Biolabs' LC-MS protein quantification service can provide you with high-precision protein quantification data. We specialize in highly sensitive quantitative analysis, accurately measuring the relative or absolute concentration of low-abundance proteins to support your research with in-depth analytical insights.
Our LC-MS Protein Quantification Service offers the following capabilities:
1. Absolute Quantification
Using external or internal standards, we accurately measure the absolute concentration of target proteins. This approach is critical for drug development and clinical research, providing essential data for drug concentration monitoring, efficacy evaluation, and toxicity studies.
2. Relative Quantification
We compare protein expression differences across different samples, uncovering variations under various conditions. This method is commonly applied to analyze protein expression differences between treatments, disease states, or species.
3. High-Sensitivity Quantification of Low-Abundance Proteins
For low-abundance protein quantification, we utilize highly sensitive mass spectrometry techniques to detect trace amounts of proteins. This meets the demands of clinical samples, exogenous proteins, or small molecule biomarker quantification.
4. Proteomics Analysis
LC-MS technology extends beyond single-protein quantification to comprehensive analysis of complex proteomes. By systematically quantifying the entire proteome, MtoZ Biolabs provides in-depth proteomic insights, aiding in the identification of potential biomarkers and associated biological pathways.
Why Choose MtoZ Biolabs?
1. High Sensitivity and Precision
Utilizing advanced Orbitrap mass spectrometer systems and Nano-LC technology, MtoZ Biolabs delivers ultra-sensitive protein quantification, detecting proteins at concentrations as low as picomolar levels. Our quantification results are highly accurate, with error margins typically within 5%.
2. Expertise in Complex Sample Handling
LC-MS efficiently separates proteins in complex samples, eliminating interferences common in traditional methods. Whether working with cell lysates, serum, tissue extracts, or other biological samples, MtoZ Biolabs provides stable and reliable quantification results.
3. High-Throughput Analysis
Our LC-MS protein quantification service can simultaneously analyze hundreds or even thousands of proteins, offering efficient support for large-scale screening and batch analysis. This is particularly valuable in drug development, disease research, and basic scientific studies.
4. Quantitative Accuracy and Flexibility
Whether you require absolute or relative quantification, our services offer flexible options. Depending on your experimental needs, we can employ labeled methods (e.g., TMT, iTRAQ) or label-free quantification, catering to diverse research backgrounds.
5. In-Depth Data Mining and Interpretation
Beyond providing basic quantitative data, MtoZ Biolabs delivers comprehensive data analysis and biological interpretation. By comparing data with databases and analyzing protein expression patterns in conjunction with pathway analyses, we provide valuable biological insights to enhance your research.
6. Comprehensive Sample Processing and Optimization
Tailored sample preparation solutions are offered for various sample types. From sample extraction and digestion to loading and analysis, every step is optimized to ensure the highest accuracy and reliability in results.
Sample Submission Suggestions
1. Sample Types
Our LC-MS protein quantification service supports various sample types, including but not limited to:
2. Sample Amount
Liquid Samples: A minimum of 50–100 μL is recommended to ensure sufficient sample volume and analytical accuracy.
Solid Samples: For tissue or cell samples, a minimum of 10 mg is recommended.
3. Sample Purity
To ensure accurate analytical results, samples should undergo appropriate purification processes. Samples containing significant impurities or interfering substances (e.g., lipids, RNA) may compromise the sensitivity of mass spectrometry analysis. Prior purification is strongly recommended in such cases.
Note: If you have specific requirements or need assistance with sample preparation, please feel free to contact us. We are here to help!
Applications
1. Drug Development
LC-MS protein quantification service plays a crucial role in drug development by supporting drug target validation, efficacy evaluation, and toxicity assessment. By quantifying changes in protein expression under drug influence, it helps developers better understand the mechanism of action of potential therapeutics.
2. Clinical Research and Biomarker Discovery
LC-MS protein quantification supports biomarker discovery, particularly in the analysis of clinical samples such as serum, urine, and tissue. It enables precise quantification of low-abundance proteins associated with diseases, providing critical data for early diagnosis and personalized treatment.
3. Basic Research
In fundamental studies, LC-MS protein quantification service helps researchers systematically analyze protein expression patterns in various biological samples. This approach uncovers essential information about biological pathways, protein-protein interactions, and cellular signaling networks.
4. Proteomics Research
Through comprehensive proteome-wide quantification using LC-MS, researchers gain insights into intracellular protein expression under different conditions, enabling the identification of proteomic differences and providing robust technical support for scientific investigations.
Case Study
Quantitative Analysis of Protein Drugs Using Immunocapture and LC-MS
Protein-based drugs, such as monoclonal antibodies and fusion proteins, hold a critical position in biopharmaceuticals. Their quantitative analysis is essential for drug development, efficacy evaluation, and quality control. Traditional enzymatic digestion coupled with mass spectrometry often faces challenges related to sensitivity and specificity when analyzing protein drugs in complex biological matrices. In this study, a strategy combining immunocapture with LC-MS protein quantification was employed. The target protein was selectively enriched using specific antibodies, followed by enzymatic digestion and LC-MS detection. Samples were processed by immunocapture to isolate the target protein, digested into peptides, and analyzed using liquid chromatography for separation and mass spectrometry for quantification. Immunocapture significantly improved the detection sensitivity and specificity of target proteins in complex biological matrices. This method enabled the precise quantification of low-abundance protein drugs in challenging samples, such as plasma, and validated the applicability of immunocapture combined with LC-MS for pharmacokinetic analysis of protein drugs.
Duggan, J. et al. Bioanalysis. 2016.
Figure2. Chromatogram of an LLOQ Sample Derived from Specificity/Selectivity Testing Showing
both IS and Analyte Integrated SRM Chromatograms
What Could be Included in the Report?
How to order?
