LFQ Proteomics Service

Proteomics has become a key technology in biological research for analyzing protein expression, interactions, and sequence modifications. Quantitative bottom-up proteomics is dominated by three different approaches: in vivo metabolic labeling, in vitro labeling, and label-free methods. In label-free quantification (LFQ) , no isotopes or labels are added to the sample, allowing for the relative quantification of proteolytic peptides through mass spectrometry. This approach typically requires fewer experimental steps and enables differential expression analysis across multiple samples simultaneously. Currently, label-free quantification has been widely applied in biomarker discovery, drug development, and disease mechanism research. The workflow of LFQ proteomics service consists of four main steps: (1) validation of database search results; (2) LC-MS/MS quantification of identified peptides; (3) LC-MS/MS quantification of unidentified peptides; and (4) protein abundance and ratio calculation.

 



Zhang, W. et al. Proteomics, 2012.

Figure 1. LFQ Workflow.

 

Services at MtoZ Biolabs

Leveraging liquid chromatography-mass spectrometry (LC-MS) technology, MtoZ Biolabs provides LFQ proteomics service to facilitate rapid and accurate measurement of the relative abundance of proteins by analyzing proteolytic peptides through mass spectrometry.

 

Service Advantages

1. Professional Technical Team

Our team members have extensive experience in proteomics research and are highly proficient LFQ proteomics service’s all aspects.

 

2. Advanced Instrumentation

We utilize high-end mass spectrometry platforms to ensure high-quality spectral data for accurate protein quantification.

 

3. Short Experimental Turnaround

Our optimized workflow enables label-free quantification experiments to be completed in a shorter timeframe.

 

4. Comprehensive Experimental Reports

Our reports provide in-depth and well-organized experimental results, offering clients a complete and detailed dataset.

 

Applications

1. Biomarker Discovery

By comparing protein expression differences in disease-related samples, LFQ proteomics service helps identify potential biomarkers, aiding in early disease diagnosis and treatment strategies.

 

2. Drug Development

Label-free quantification can assess the effects of drugs on protein expression, providing insights into drug mechanisms of action and potential side effects.

 

3. Disease Mechanism Research

LFQ proteomics service enables researchers to explore protein expression changes during disease progression, offering a deeper understanding of disease pathophysiology.

 

4. Biological Research

Label-free quantification helps study protein expression changes under different physiological states, revealing biological processes and signaling pathways.

 

Case Study

1. LFQ of Inosine Phosphate in Drosophila Melanogaster by Mass Spectrometry

Phosphoinositides (PIs) are seven phosphorylated derivatives of phosphatidylinositol, considered key molecules regulating multiple molecular events in eukaryotic cells. PIs are low-abundance lipids, making their detection and quantification particularly challenging. While many methods allow for PI quantification through radioactive labeling in cultured cells, these approaches are not applicable in in vivo animal models, which are essential for studying cellular and developmental processes. Currently, label-free quantification techniques standardize biological sample size differences, aiding in the investigation of how PIs regulate complex cellular processes and development in higher eukaryotic organisms.

 



Ghosh, A. et al. Phosphoinositides: Methods and Protocols, 2021.

Figure 2. Analysis of Typical Lipid Extraction Process of Inosine Phosphate by LC-MS/MS.

 

Deliverables

1. Label-Free Quantification Results

 

2. Bioinformatics Analysis

 

3. Raw Data Files

 

4. Experimental Report

 

MtoZ Biolabs, an integrated Chromatography and Mass Spectrometry (MS) Services Provider, provides advanced proteomics, metabolomics, and biopharmaceutical analysis services to researchers in biochemistry, biotechnology, and biopharmaceutical fields. Our ultimate aim is to provide more rapid, high-throughput, and cost-effective analysis, with exceptional data quality and minimal sample consumption. Free project evaluation, welcome to learn more details!